Microbe-derived Antioxidants Enhance Lipid Synthesis by Regulating the Hepatic AMPKα–SREBP1c Pathway in Weanling Piglets

• Published in: The Journal of nutrition 2024-04, Vol.154 (4), p.1101-1108
• Main Authors: Yu, Chengbing, Wang, Di, Shen, Cheng, Luo, Zhen, Zhang, Hongcai, Zhang, Jing, Xu, Weina, Xu, Jianxiong
• Format: Article
• Language: English
• Subjects: Adenosine kinase; Adenosine monophosphate; Age; AMP; AMPKα–SREBP1c pathway; Animals; Antioxidants; Antioxidants - metabolism; Cholesterol; Fatty-acid synthase; Ghrelin; Hogs; Kinases; Leptin; Lipase; Lipid Metabolism; lipid synthesis; Lipids; Lipolysis; Liver; Low density lipoprotein; Metabolism; microbe-derived antioxidants; Microorganisms; Nutrition; Peroxisome proliferator-activated receptors; Phosphorylation; Protein kinase; Protein Kinases - metabolism; Proteins; Sterol Regulatory Element Binding Protein 1 - metabolism; Swine; Synthesis; Triglycerides; Weaning; weanling piglets; Weight loss
• ISSN: 0022-3166; 1541-6100; 1541-6100
• DOI: 10.1016/j.tjnut.2024.02.002

Weaning usually causes low feed intake and weight loss in piglets, which mobilizes lipid to energize. The microbe-derived antioxidants (MAs) exhibit great potential in antioxidation, anti-inflammation, and metabolic regulation. We aimed to investigate the changes of lipid metabolism postweaning and effects of MA on growth performance and hepatic lipid metabolism in weanling piglets. In the first experiment, piglets weaned at 21 d of age were slaughtered on weaning day (d0), 4 (d4), and 14 (d14) postweaning (6 piglets per day). In the second experiment, piglets were divided into 2 groups, receiving MA (MA) and saline gavage (CON), respectively. All piglets were weaned at 21 d of age and 6 piglets from each group were slaughtered at 25 d of age. In experiment 1, the serum triglyceride, total cholesterol (TC), and LDL cholesterol on d4 and d14 declined significantly compared with d0 (P < 0.05). The serum leptin on d0 was higher than that on d4 and d14 (P < 0.05). The serum ghrelin kept increasing from d0 to d14 (P < 0.05). The hepatic hormone-sensitive lipase and adipose triglyceride lipase first increased from d0 to d4 and then decreased from d4 to d14 (P < 0.05). In experiment 2, the average daily gain and average daily feed intake from 21 to 25 d of age increased in the MA group compared with the CON group (P < 0.05). The serum TC, hepatic TC, and glucose of MA group showed a significant increase than that of the CON group (P < 0.05). The expression of SCD1, ACAT2, and PPARγ were upregulated in the MA group (P < 0.05). Contrary to the decreased expression of phosphorylation of adenosine 5′-monophosphate-activated protein kinase alfa subunit (Thr172), the nuclear sterol regulatory element–binding protein 1c, fatty acid synthase, and peroxisome proliferator-activated receptor gamma of MA group increased than that of CON group (P < 0.05). Weaning promoted hepatic lipolysis and MA could enhance lipid synthesis by regulating adenosine 5′-monophosphate-activated protein kinase alfa subunit–sterol regulatory element–binding protein 1c pathway, thus improving growth performance of weanling piglets.