RTN3 deficiency exacerbates cisplatin-induced acute kidney injury through the disruption of mitochondrial stability

• Published in: Mitochondrion 2024-03, Vol.75, p.101851-101851, Article 101851
• Main Authors: Du, Ran, Liu, Ji-Shi, Huang, Hao, Liu, Yu-Xing, Jin, Jie-Yuan, Wang, Chen-Yu, Dong, Yi, Fan, Liang-Liang, Xiang, Rong
• Format: Article
• Language: English
• Subjects: AKI; HSPA9; Mitochondria-associated membranes; Mitochondrial dysfunction; RTN3
• ISSN: 1567-7249; 1872-8278
• DOI: 10.1016/j.mito.2024.101851

•We may first reveal that RTN3 deficiency can exacerbate cisplatin-induced acute kidney injury.•RTN3 deficiency can disrupt mitochondrial function and promotes cell apoptosis after cisplatin treatment.•RTN3 participates in mitochondrial homeostasis via interact with HSPA9. Reticulum 3 (RTN3) is an endoplasmic reticulum (ER) protein that has been reported to act in neurodegenerative diseases and lipid metabolism. However, the role of RTN3 in acute kidney injury (AKI) has not been explored. Here, we employed public datasets, patient data, and animal models to explore the role of RTN3 in AKI. The underlying mechanisms were studied in primary renal tubular epithelial cells and in the HK2 cell line. We found reduced expression of RTN3 in AKI patients, cisplatin-induced mice, and cisplatin-treated HK2 cells. RTN3-null mice exhibit more severe AKI symptoms and kidney fibrosis after cisplatin treatment. Mitochondrial dysfunction was also found in cells with RTN3 knockdown or knockout. A mechanistic study revealed that RTN3 can interact with HSPA9 in kidney cells. RTN3 deficiency may disrupt the RTN3–HSPA9–VDAC2 complex and affect MAMs during ER–mitochondrion contact, which further leads to mitochondrial dysfunction and exacerbates cisplatin-induced AKI. Our study indicated that RTN3 was important in the kidney and that a decrease in RTN3 in the kidney might be a risk factor for the aggravation of AKI.