Flow-Through-Type DNA Array Based on Ideally Ordered Anodic Porous Alumina Substrate

A flow‐through‐type DNA array fabricated from an ideally ordered anodic porous alumina substrate is reported. Single‐stranded probe DNA was affixed to the walls of the sub‐micrometer‐diameter holes, and fluorescently labelled target DNA was passed through the holes in the substrate. Fluorescence mic...

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Published in:Advanced materials (Weinheim) 2004-12, Vol.16 (23-24), p.2105-2108
Main Authors: Matsumoto, F., Nishio, K., Masuda, H.
Format: Article
Language:English
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Alumina
DNA
Fluorescence
Membranes
Nanoporous materials
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description A flow‐through‐type DNA array fabricated from an ideally ordered anodic porous alumina substrate is reported. Single‐stranded probe DNA was affixed to the walls of the sub‐micrometer‐diameter holes, and fluorescently labelled target DNA was passed through the holes in the substrate. Fluorescence microscopy revealed an ordered array of fluorescent spots after the hybridization (see Figure), demonstrating the applicability of these porous alumina substrates to fabrication of high‐density DNA arrays.
doi_str_mv 10.1002/adma.200400360
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subjects Alumina
DNA
Fluorescence
Membranes
Nanoporous materials
title Flow-Through-Type DNA Array Based on Ideally Ordered Anodic Porous Alumina Substrate
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