Volatile Organic Compounds from Offspring of Stingless Bee Sacrificed in Hygienic Behavior Test

This study shows the profile of volatile organic compounds (VOCs) from pupae and larvae of Melipona quadrifasciata anthidioides Lepeletier subjected to three death induction techniques for hygienic behavior (HB) studies: freezing in liquid nitrogen (LN2), freezing in a freezer (FRZ) and piercing of...

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Bibliographic Details
Published in:Chemistry & biodiversity 2024-03, Vol.21 (3), p.e202301641-n/a
Main Authors: Neiva de Jesus, Jossimara, Ribeiro Mesquita, Paulo Roberto, Barbosa da Silva, Kelly, Medeiros Rodrigues, Frederico, Lopes de Carvalho, Carlos Alfredo, Gomes da Costa, João, Lima Aguiar, Cândida Maria
Format: Article
Language:English
Subjects:
Alcohols
Bee health
Bees
Colonies
Dead brood removal behavior
Developmental stages
Freezing
Gas chromatography
GC-MS
Headspace
Hygiene
Larvae
Liquid nitrogen
Mass spectrometry
Mass spectroscopy
Multivariate analysis
Offspring
Organic compounds
Pupae
Stingless bees
Terpenes
VOCs
Volatile organic compounds
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Summary:This study shows the profile of volatile organic compounds (VOCs) from pupae and larvae of Melipona quadrifasciata anthidioides Lepeletier subjected to three death induction techniques for hygienic behavior (HB) studies: freezing in liquid nitrogen (LN2), freezing in a freezer (FRZ) and piercing of offspring with an entomological pin (PIN). The VOCs from larvae and pupae were obtained through headspace solid‐phase microextraction and characterized using gas chromatography coupled to mass spectrometry. In addition, an HB test was performed on the colonies. The main classes of VOCs were hydrocarbons, terpenes and alcohols. Multivariate analysis was applied and showed that there was a separation in the compound profiles between the different treatments. The HB test in the colonies showed that 24 hours after the application of the techniques, the bees removed more dead larvae in LN2 treatment (83.5 %), while after 48 hours more larvae were removed in the LN2 and FRZ treatments (92.3 %). When compared to pupae removal, larvae removal was significantly faster in LN2.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202301641