Magnetite alleviating calcification of anaerobic granular sludge (AnGS): Electron transfer enhancement and ion competition

• Published in: The Science of the total environment 2024-04, Vol.920, p.170981-170981, Article 170981
• Main Authors: Chen, Linlin, Yuan, Ruoxuan, Xu, Xiangyang, Zhu, Liang
• Format: Article
• Language: English
• Subjects: Anaerobic granular sludge; Calcification; Extracellular polymeric substances; Ion competition; Magnetite
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.170981

Calcification accompanied by deactivation of anaerobic granular sludge (AnGS) is a continuing challenge for high calcium wastewater treatment. The interaction between Ca2+ and extracellular polymeric substances (EPS) is a precondition for this problem. In this study, magnetite for activity recovery and calcification alleviation simultaneously of AnGS under high calcium stress was investigated. The results showed that, in the presence of magnetite, the relative biogas production increased by 13.2 % with the higher activities of key enzymes involved in methanogenesis. Methanosarcina turned into the dominant methanogens, and syntrophic bacteria such as Chloroflexi, Synergistota were enriched, which indicated the enhancement of electron transfer by magnetite, supported by an 18 % increase of the electron transfer system (ETS) activity. Further characterizations of AnGS suggested that the granule calcification was alleviated with a final decrease of 13–40 % calcium content of AnGS with particle size of 1–2.5 mm. Besides, calcium was partially substituted by iron in the EPS, and the secretion of EPS especially proteins decreased. Batch tests demonstrated the competition between Fe2+ dissolved from magnetite and Ca2+, which interfered the interaction between Ca2+ and EPS, so the granule calcification was prevented. Therefore, magnetite played a pluripotent role in the alleviation of granule calcification and deactivation in situ via (1) enhancing electron transfer, and (2) blocking the complex between Ca2+ and EPS. This study provides a novel insight into the application of conductive metal materials in biological wastewater treatment systems suffering from high calcium attack. [Display omitted] •Magnetite for activity recovery and calcification alleviation simultaneously of AnGS was achieved.•Methanosarcina substituted for Methanosaeta and Methanobacterium as the dominant methanogens.•Magnetite alleviating calcification with a decrease of 13–40 % calcium content of AnGS.•Fe2+ released from magnetite competed with Ca2+ for the complex with EPS.