Enhanced bioremediation of acid mine-influenced groundwater with micro-sized emulsified corn oil droplets (MOD) and sulfate-reducing bacteria (Desulfovibrio vulgaris) in a microcosm assay

• Published in: Chemosphere (Oxford) 2024-03, Vol.352, p.141403-141403, Article 141403
• Main Authors: Hussain, Fida, Kim, Lan Hee, Kim, Huiyun, Kim, Young, Oh, Sang-Eun, Kim, Sungpyo
• Format: Article
• Language: English
• Subjects: Acid mine drainage; Acids; Bacteria; Bio-precipitation; Biodegradation, Environmental; Carbon; Corn Oil; Desulfovibrio; Desulfovibrio vulgaris; Ecosystem; Heavy metals; Metal sulfide; Metals, Heavy; Sulfate-reducing bacteria; Sulfates; Sulfides; Zea mays
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.141403

High concentrations of metals and sulfates in acid mine drainage (AMD) are the cause of the severe environmental hazard that mining operations pose to the surrounding ecosystem. Little study has been conducted on the cost-effective biological process for treating high AMD. The current research investigated the potential of the proposed carbon source and sulfate reduction bacteria (SRB) culture in achieving the bioremediation of sulfate and heavy metals. This work uses individual and combinatorial bioaugmentation and bio-stimulation methods to bioremediate acid-mine-influenced groundwater in batch microcosm experiments. Bioaugmentation and bio-stimulation methods included pure culture SRB (Desulfovibrio vulgaris) and microsized oil droplet (MOD) by emulsifying corn oil. The research tested natural attenuation (T 1), bioaugmentation (T2), biostimulation (T3), and bioaugmentation plus biostimulation (T4) for AM-contaminated groundwater remediation. Bioaugmentation and bio-stimulation showed the greatest sulfate reduction (75.3%) and metal removal (95–99%). Due to carbon supply scarcity, T1 and T2 demonstrated 15.7% and 27.8% sulfate reduction activities. Acetate concentrations in T3 and T4 increased bacterial activity by providing carbon sources. Metal bio-precipitation was substantially linked with sulfate reduction and cell growth. SEM-EDS study of precipitates in T3 and T4 microcosm spectra indicated peaks for S, Cd, Mn, Cu, Zn, and Fe, indicating metal-sulfide association for metal removal precipitates. The MOD provided a constant carbon source for indigenous bacteria, while Desulfovibrio vulgaris increased biogenic sulfide synthesis for heavy metal removal. [Display omitted] •Bioaugmentation and bio-stimulation approach to remediate acid mine drainage (AMD) influenced groundwater.•Microsized oil droplets (MOD) based Emulsified corn oil was used as a carbon source.•High removal of heavy metals and sulfate have been achieved.•Metal bio-precipitation was linked with sulfate reduction and SRB cell growth.