Voltage-gated sodium channel gene mutation and P450 gene expression are associated with the resistance of Aphis spiraecola Patch (Hemiptera: Aphididae) to lambda-cyhalothrin

• Published in: Bulletin of entomological research 2024-02, Vol.114 (1), p.49-56
• Main Authors: Tang, Hong-Cheng, Zhou, Yu-Rong, Zuo, Jun-Feng, Wang, Yi-Xuan, Piñero, Jaime C, Peng, Xiong, Chen, Mao-Hua
• Format: Article
• Language: English
• ISSN: 0007-4853; 1475-2670
• DOI: 10.1017/S0007485323000603

Patch is one of the most economically important tree fruit pests worldwide. The pyrethroid insecticide lambda-cyhalothrin is commonly used to control . In this 2-year study, we quantified the resistance level of to lambda-cyhalothrin in different regions of the Shaanxi province, China. The results showed that had reached extremely high resistance levels with a 174-fold resistance ratio (RR) found in the Xunyi region. In addition, we compared the enzymatic activity and expression level of P450 genes among eight populations. The P450 activity of was significantly increased in five regions (Xunyi, Liquan, Fengxiang, Luochuan, and Xinping) compared to susceptible strain (SS). The expression levels of , , , , , and genes were significantly increased under lambda-cyhalothrin treatment and in the resistant field populations. A L1014F mutation in the sodium channel gene was found and the mutation rate was positively correlated with the LC of lambda-cyhalothrin. In conclusion, the levels of lambda-cyhalothrin resistance of field populations were associated with P450s and L1014F mutations. Our combined findings provide evidence on the resistance mechanism of to lambda-cyhalothrin and give a theoretical basis for rational and effective control of this pest species.