Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study

Objective The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures. Materials and methods Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa,...

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Bibliographic Details
Published in:Journal of orofacial orthopedics 2024-08, Vol.85 (Suppl 2), p.155-164
Main Authors: Bachiega Morelli, Raquel Fernanda, Nelson-Filho, Paulo, Carpio Horta, Karla, Feres, Magda, Lima Ferreira, José Tarcísio, Romano, Fábio Lourenço, Sasso Stuani, Maria Bernadete, Pereira Saraiva, Maria Conceição, Bitencourt Reis, Caio Luiz, Nakane Matsumoto, Mirian Aiko
Format: Article
Language:English
Subjects:
Adolescent
Child
Dentistry
Elastomers
Equipment Contamination
Female
Humans
Male
Medicine
Nucleic Acid Hybridization
Oral and Maxillofacial Surgery
Original Article
Orthodontic Appliances - microbiology
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Summary:Objective The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures. Materials and methods Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model. Results The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups ( p  > 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups ( p  > 0.05). Conclusions Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.
ISSN:1434-5293
1615-6714
1615-6714
DOI:10.1007/s00056-023-00507-w