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Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study
Objective The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures. Materials and methods Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa,...
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| Published in: | Journal of orofacial orthopedics 2024-08, Vol.85 (Suppl 2), p.155-164 |
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| Main Authors: | , , , , , , , , , |
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| Language: | English |
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| container_end_page | 164 |
| container_issue | Suppl 2 |
| container_start_page | 155 |
| container_title | Journal of orofacial orthopedics |
| container_volume | 85 |
| creator | Bachiega Morelli, Raquel Fernanda Nelson-Filho, Paulo Carpio Horta, Karla Feres, Magda Lima Ferreira, José Tarcísio Romano, Fábio Lourenço Sasso Stuani, Maria Bernadete Pereira Saraiva, Maria Conceição Bitencourt Reis, Caio Luiz Nakane Matsumoto, Mirian Aiko |
| description | Objective
The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.
Materials and methods
Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.
Results
The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (
p
> 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (
p
> 0.05).
Conclusions
Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands. |
| doi_str_mv | 10.1007/s00056-023-00507-w |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2928946610</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2928946610</sourcerecordid><originalsourceid>FETCH-LOGICAL-c298t-48c75a90835a22bd0cdf614392afd3016275cd792278d6c0fb154b523280b99b3</originalsourceid><addsrcrecordid>eNp9kE1u1zAQxS1ERUvhAiyqLLsJjO3EsZdVWz6klm5gbdnO5B-XJG5tR1URi96BG3ISXFJYsvE8yW_e6P0IeUPhLQXo3iUAaEUNjNdFQFffPSMHVNC2Fh1tnhfd8KZumeL75GVK14_2BsQLss8lldBIfkB-XHoXg_VmqlxYspn9YrIPS3UTw-AnrIrElEfM3lU4mZTDjLHoye9MXiOmKo8xrLuxTKzciO4bRhtM7Kuzzye_Hn6WtxrvbfS9_75FZ3Tj4m9XfEX2BjMlfP00D8nX9-dfTj_WF1cfPp2eXNSOKZnrRrquNQokbw1jtgfXD6J0U8wMPQcqWNe6vlOMdbIXDgZL28a2jDMJVinLD8nxlltKlbMp69knh9NkFgxr0kwxqRohKBQr26yFSkoRB30T_WzivaagH6nrjbou1PUf6vquLB095a92xv7fyl_MxcA3Qypfyw6jvg5rXErn_8X-BsfFkKM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2928946610</pqid></control><display><type>article</type><title>Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study</title><source>Springer Nature</source><creator>Bachiega Morelli, Raquel Fernanda ; Nelson-Filho, Paulo ; Carpio Horta, Karla ; Feres, Magda ; Lima Ferreira, José Tarcísio ; Romano, Fábio Lourenço ; Sasso Stuani, Maria Bernadete ; Pereira Saraiva, Maria Conceição ; Bitencourt Reis, Caio Luiz ; Nakane Matsumoto, Mirian Aiko</creator><creatorcontrib>Bachiega Morelli, Raquel Fernanda ; Nelson-Filho, Paulo ; Carpio Horta, Karla ; Feres, Magda ; Lima Ferreira, José Tarcísio ; Romano, Fábio Lourenço ; Sasso Stuani, Maria Bernadete ; Pereira Saraiva, Maria Conceição ; Bitencourt Reis, Caio Luiz ; Nakane Matsumoto, Mirian Aiko</creatorcontrib><description>Objective
The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.
Materials and methods
Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.
Results
The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (
p
> 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (
p
> 0.05).
Conclusions
Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.</description><identifier>ISSN: 1434-5293</identifier><identifier>ISSN: 1615-6714</identifier><identifier>EISSN: 1615-6714</identifier><identifier>DOI: 10.1007/s00056-023-00507-w</identifier><identifier>PMID: 38180483</identifier><language>eng</language><publisher>Heidelberg: Springer Medizin</publisher><subject>Adolescent ; Child ; Dentistry ; Elastomers ; Equipment Contamination ; Female ; Humans ; Male ; Medicine ; Nucleic Acid Hybridization ; Oral and Maxillofacial Surgery ; Original Article ; Orthodontic Appliances - microbiology</subject><ispartof>Journal of orofacial orthopedics, 2024-08, Vol.85 (Suppl 2), p.155-164</ispartof><rights>The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature 2024</rights><rights>2024. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c298t-48c75a90835a22bd0cdf614392afd3016275cd792278d6c0fb154b523280b99b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38180483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bachiega Morelli, Raquel Fernanda</creatorcontrib><creatorcontrib>Nelson-Filho, Paulo</creatorcontrib><creatorcontrib>Carpio Horta, Karla</creatorcontrib><creatorcontrib>Feres, Magda</creatorcontrib><creatorcontrib>Lima Ferreira, José Tarcísio</creatorcontrib><creatorcontrib>Romano, Fábio Lourenço</creatorcontrib><creatorcontrib>Sasso Stuani, Maria Bernadete</creatorcontrib><creatorcontrib>Pereira Saraiva, Maria Conceição</creatorcontrib><creatorcontrib>Bitencourt Reis, Caio Luiz</creatorcontrib><creatorcontrib>Nakane Matsumoto, Mirian Aiko</creatorcontrib><title>Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study</title><title>Journal of orofacial orthopedics</title><addtitle>J Orofac Orthop</addtitle><addtitle>J Orofac Orthop</addtitle><description>Objective
The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.
Materials and methods
Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.
Results
The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (
p
> 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (
p
> 0.05).
Conclusions
Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.</description><subject>Adolescent</subject><subject>Child</subject><subject>Dentistry</subject><subject>Elastomers</subject><subject>Equipment Contamination</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medicine</subject><subject>Nucleic Acid Hybridization</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original Article</subject><subject>Orthodontic Appliances - microbiology</subject><issn>1434-5293</issn><issn>1615-6714</issn><issn>1615-6714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1u1zAQxS1ERUvhAiyqLLsJjO3EsZdVWz6klm5gbdnO5B-XJG5tR1URi96BG3ISXFJYsvE8yW_e6P0IeUPhLQXo3iUAaEUNjNdFQFffPSMHVNC2Fh1tnhfd8KZumeL75GVK14_2BsQLss8lldBIfkB-XHoXg_VmqlxYspn9YrIPS3UTw-AnrIrElEfM3lU4mZTDjLHoye9MXiOmKo8xrLuxTKzciO4bRhtM7Kuzzye_Hn6WtxrvbfS9_75FZ3Tj4m9XfEX2BjMlfP00D8nX9-dfTj_WF1cfPp2eXNSOKZnrRrquNQokbw1jtgfXD6J0U8wMPQcqWNe6vlOMdbIXDgZL28a2jDMJVinLD8nxlltKlbMp69knh9NkFgxr0kwxqRohKBQr26yFSkoRB30T_WzivaagH6nrjbou1PUf6vquLB095a92xv7fyl_MxcA3Qypfyw6jvg5rXErn_8X-BsfFkKM</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Bachiega Morelli, Raquel Fernanda</creator><creator>Nelson-Filho, Paulo</creator><creator>Carpio Horta, Karla</creator><creator>Feres, Magda</creator><creator>Lima Ferreira, José Tarcísio</creator><creator>Romano, Fábio Lourenço</creator><creator>Sasso Stuani, Maria Bernadete</creator><creator>Pereira Saraiva, Maria Conceição</creator><creator>Bitencourt Reis, Caio Luiz</creator><creator>Nakane Matsumoto, Mirian Aiko</creator><general>Springer Medizin</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240801</creationdate><title>Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique</title><author>Bachiega Morelli, Raquel Fernanda ; Nelson-Filho, Paulo ; Carpio Horta, Karla ; Feres, Magda ; Lima Ferreira, José Tarcísio ; Romano, Fábio Lourenço ; Sasso Stuani, Maria Bernadete ; Pereira Saraiva, Maria Conceição ; Bitencourt Reis, Caio Luiz ; Nakane Matsumoto, Mirian Aiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-48c75a90835a22bd0cdf614392afd3016275cd792278d6c0fb154b523280b99b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adolescent</topic><topic>Child</topic><topic>Dentistry</topic><topic>Elastomers</topic><topic>Equipment Contamination</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medicine</topic><topic>Nucleic Acid Hybridization</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original Article</topic><topic>Orthodontic Appliances - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bachiega Morelli, Raquel Fernanda</creatorcontrib><creatorcontrib>Nelson-Filho, Paulo</creatorcontrib><creatorcontrib>Carpio Horta, Karla</creatorcontrib><creatorcontrib>Feres, Magda</creatorcontrib><creatorcontrib>Lima Ferreira, José Tarcísio</creatorcontrib><creatorcontrib>Romano, Fábio Lourenço</creatorcontrib><creatorcontrib>Sasso Stuani, Maria Bernadete</creatorcontrib><creatorcontrib>Pereira Saraiva, Maria Conceição</creatorcontrib><creatorcontrib>Bitencourt Reis, Caio Luiz</creatorcontrib><creatorcontrib>Nakane Matsumoto, Mirian Aiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orofacial orthopedics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bachiega Morelli, Raquel Fernanda</au><au>Nelson-Filho, Paulo</au><au>Carpio Horta, Karla</au><au>Feres, Magda</au><au>Lima Ferreira, José Tarcísio</au><au>Romano, Fábio Lourenço</au><au>Sasso Stuani, Maria Bernadete</au><au>Pereira Saraiva, Maria Conceição</au><au>Bitencourt Reis, Caio Luiz</au><au>Nakane Matsumoto, Mirian Aiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study</atitle><jtitle>Journal of orofacial orthopedics</jtitle><stitle>J Orofac Orthop</stitle><addtitle>J Orofac Orthop</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>85</volume><issue>Suppl 2</issue><spage>155</spage><epage>164</epage><pages>155-164</pages><issn>1434-5293</issn><issn>1615-6714</issn><eissn>1615-6714</eissn><abstract>Objective
The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.
Materials and methods
Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.
Results
The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (
p
> 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (
p
> 0.05).
Conclusions
Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.</abstract><cop>Heidelberg</cop><pub>Springer Medizin</pub><pmid>38180483</pmid><doi>10.1007/s00056-023-00507-w</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 1434-5293 |
| ispartof | Journal of orofacial orthopedics, 2024-08, Vol.85 (Suppl 2), p.155-164 |
| issn | 1434-5293 1615-6714 1615-6714 |
| language | eng |
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| source | Springer Nature |
| subjects | Adolescent Child Dentistry Elastomers Equipment Contamination Female Humans Male Medicine Nucleic Acid Hybridization Oral and Maxillofacial Surgery Original Article Orthodontic Appliances - microbiology |
| title | Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique: A randomized split-mouth study |
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