Isolation and whole‐genome sequencing analysis of Escherichia fergusonii harboring a heat‐labile enterotoxin gene from retail chicken meat in Okinawa, Japan

This study aimed to reveal the prevalence of heat‐labile enterotoxin (LT) gene‐positive Escherichia fergusonii in retail chicken meat and genetically characterize these strains. E. fergusonii harboring LT gene was isolated from 6 out of 60 (10%) retail chicken samples in Okinawa, Japan. Whole‐genome...

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Bibliographic Details
Published in:Microbiology and immunology 2024-03, Vol.68 (3), p.115-121
Main Authors: Kakita, Tetsuya, Lee, Kenichi, Morita, Masatomo, Okuno, Miki, Kyan, Hisako, Okano, Sho, Maeshiro, Noriyuki, Ishizu, Momoko, Kudeken, Tsuyoshi, Taira, Haruno, Teruya, Morimi, Ogura, Yoshitoshi, Akeda, Yukihiro, Ohnishi, Makoto
Format: Article
Language:English
Subjects:
Escherichia
Escherichia fergusonii
Genomes
heat‐labile enterotoxin
Japan
LT gene
Meat
Multidrug resistance
Pathogenicity
Plasmids
retail chicken meat
Sequence analysis
whole‐genome sequencing
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Summary:This study aimed to reveal the prevalence of heat‐labile enterotoxin (LT) gene‐positive Escherichia fergusonii in retail chicken meat and genetically characterize these strains. E. fergusonii harboring LT gene was isolated from 6 out of 60 (10%) retail chicken samples in Okinawa, Japan. Whole‐genome sequencing analysis revealed that LT gene‐positive E. fergusonii from chicken meat and feces contain an IncFII plasmid harboring elt1AB, and suggested to spread clonally to retail chicken through fecal contamination. Additionally, it was found that these strains harbor multidrug‐resistant genes on their plasmids. Their pathogenicity and continuous monitoring are required for confirmation.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.13115