Optimizing chemical-induced premature chromosome condensation assay for rapid estimation of high-radiation doses

In the event of exposure to high doses of radiation, prompt dose estimation is crucial for selecting appropriate treatment modalities, such as cytokine therapy or stem cell transplantation. The chemical-induced premature chromosome condensation (PCC) method offers a simple approach for such dose est...

Full description

Saved in:
Bibliographic Details
Published in:Radiation protection dosimetry 2024-04, Vol.200 (5), p.448-458
Main Authors: Nakayama, Ryo, Anderson, Donovan, Goh, Valerie Swee Ting, Fujishima, Yohei, Yanagidate, Kaito, Ariyoshi, Kentaro, Kasai, Kosuke, Yoshida, Mitsuaki A, Blakely, William F, Miura, Tomisato
Format: Article
Language:English
Subjects:
Caffeine
Chromosome Aberrations
Chromosomes
Dose-Response Relationship, Radiation
Humans
Lymphocytes
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the event of exposure to high doses of radiation, prompt dose estimation is crucial for selecting appropriate treatment modalities, such as cytokine therapy or stem cell transplantation. The chemical-induced premature chromosome condensation (PCC) method offers a simple approach for such dose estimation with significant radiation exposure, but its 48-h incubation time poses challenges for early dose assessment. In this study, we optimized the chemical-induced PCC assay for more rapid dose assessment. A sufficient number of PCC and G2/M-PCC cells were obtained after 40 h of culture for irradiated human peripheral blood up to 20 Gy. By adding caffeine (final concentration of 1 mM) at 34 h from the start of culture, G2/M-PCC index increased by 1.4-fold in 10 Gy cultures. There was also no significant difference in the G2/M-PCC ring frequency induced for doses 0 to 15 Gy between our 40-h caffeine-supplemented chemical-induced PCC method and the conventional 48-h PCC assay.
ISSN:0144-8420
1742-3406
DOI:10.1093/rpd/ncad312