Biocompatible N-carbazoleacetic acid decorated CuxO nanoparticles as self-cascading platforms for synergistic single near-infrared triggered phototherapy treating microbial infections

In this work, positively charged N-carbazoleacetic acid decorated CuxO nanoparticles (CuxO-CAA NPs) as novel biocompatible nanozymes have been successfully prepared through a one-step hydrothermal method. CuxO-CAA can serve as a self-cascading platform through effective GSH-OXD-like and POD-like act...

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Published in:Biomaterials science 2024-03, Vol.12 (6), p.1558-1572
Main Authors: Xiao-Chan, Yang, Ding, Yong, Sheng-Nan, Song, Wen-Hui, Wang, Huang, Shan, Xue-Yao, Pang, Li, Bo, Yu, Ya-Ya, Ya-Mu Xia, Wei-Wei, Gao
Format: Article
Language:English
Subjects:
Ampicillin
Biocompatibility
Catalytic oxidation
Cell membranes
E coli
Hydrogen peroxide
Hyperthermia
Microorganisms
Nanoparticles
Near infrared radiation
Oxidation
Photothermal conversion
Staphylococcus infections
Substrates
Wound healing
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Summary:In this work, positively charged N-carbazoleacetic acid decorated CuxO nanoparticles (CuxO-CAA NPs) as novel biocompatible nanozymes have been successfully prepared through a one-step hydrothermal method. CuxO-CAA can serve as a self-cascading platform through effective GSH-OXD-like and POD-like activities, and the former can induce continuous generation of H2O2 through the catalytic oxidation of overexpressed GSH in the bacterial infection microenvironment, which in turn acts as a substrate for the latter to yield ·OH via Fenton-like reaction, without introducing exogenous H2O2. Upon NIR irradiation, CuxO-CAA NPs possess a high photothermal conversion effect, which can further improve the enzymatic activity for increasing the production rate of H2O2 and ·OH. Besides, the photodynamic performance of CuxO-CAA NPs can produce 1O2. The generated ROS and hyperthermia have synergetic effects on bacterial mortality. More importantly, CuxO-CAA NPs are more stable and biosafe than Cu2O, and can generate electrostatic adsorption with negatively charged bacterial cell membranes and accelerate bacterial death. Antibacterial results demonstrate that CuxO-CAA NPs are lethal against methicillin-resistant Staphylococcus aureus (MRSA) and ampicillin-resistant Escherichia coli (AREC) through destroying the bacterial membrane and disrupting the bacterial biofilm formation. MRSA-infected animal wound models show that CuxO-CAA NPs can efficiently promote wound healing without causing toxicity to the organism.
ISSN:2047-4830
2047-4849
DOI:10.1039/d3bm01873c