Simultaneous Electrochemical Detection of Multiple Bacterial Species Using Metal–Organic Nanohybrids

Organic metallic nanohybrids (NHs), in which many small metal nanoparticles are encapsulated within a conductive polymer matrix, are useful as sensitive electrochemical labels because the constituents produce characteristic oxidation current responses. Gold NHs, consisting of gold nanoparticles and...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2024-03, Vol.96 (9), p.3787-3793
Main Authors: Itagaki, Satohiro, Nakao, Akihiro, Nakamura, Shogo, Fujita, Masashi, Nishii, Shigeki, Yamamoto, Yojiro, Sadanaga, Yasuhiro, Shiigi, Hiroshi
Format: Article
Language:English
Subjects:
Antibodies
Bacteria
Biosensing Techniques - methods
Conducting polymers
Copper
E coli
Electrochemical analysis
Electrochemical Techniques
Electrochemistry
Electrodes
Gold
Gold - chemistry
Labels
Limit of Detection
Metal Nanoparticles - chemistry
Nanoparticles
Oxidation
Oxidation-Reduction
Polyanilines
Polymers
Toluidine
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Summary:Organic metallic nanohybrids (NHs), in which many small metal nanoparticles are encapsulated within a conductive polymer matrix, are useful as sensitive electrochemical labels because the constituents produce characteristic oxidation current responses. Gold NHs, consisting of gold nanoparticles and poly­(m-toluidine), and copper NHs, consisting of copper nanoparticles and polyaniline, did not interfere with each other in terms of the electrochemical signals obtained on the same electrode. Antibodies were introduced into these NHs to function as electrochemical labels for targeting specific bacteria. Electrochemical measurements using screen-printed electrodes dry-fixed with NH-labeled bacterial cells enabled the estimation of bacterial species and number within minutes, based on the distinct current response of the labels. Our proposed method achieved simultaneous detection of enterohemorrhagic Escherichia coli and Staphylococcus aureus in a real sample. These NHs will be powerful tools as electrochemical labels and are expected to be useful for rapid testing in food and drug-related manufacturing sites.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.3c04587