Simultaneous Electrochemical Detection of Multiple Bacterial Species Using Metal–Organic Nanohybrids

Organic metallic nanohybrids (NHs), in which many small metal nanoparticles are encapsulated within a conductive polymer matrix, are useful as sensitive electrochemical labels because the constituents produce characteristic oxidation current responses. Gold NHs, consisting of gold nanoparticles and...

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Published in:Analytical chemistry (Washington) 2024-03, Vol.96 (9), p.3787-3793
Main Authors: Itagaki, Satohiro, Nakao, Akihiro, Nakamura, Shogo, Fujita, Masashi, Nishii, Shigeki, Yamamoto, Yojiro, Sadanaga, Yasuhiro, Shiigi, Hiroshi
Format: Article
Language:English
Subjects:
analytical chemistry
Antibodies
Bacteria
Biosensing Techniques - methods
Conducting polymers
Copper
copper nanoparticles
E coli
Electrochemical analysis
Electrochemical Techniques
Electrochemistry
Electrodes
enterohemorrhagic Escherichia coli
Gold
Gold - chemistry
Labels
Limit of Detection
Metal Nanoparticles - chemistry
nanogold
nanohybrids
Nanoparticles
Oxidation
Oxidation-Reduction
polyaniline
Polyanilines
Polymers
Staphylococcus aureus
Toluidine
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creator Itagaki, Satohiro
Nakao, Akihiro
Nakamura, Shogo
Fujita, Masashi
Nishii, Shigeki
Yamamoto, Yojiro
Sadanaga, Yasuhiro
Shiigi, Hiroshi
description Organic metallic nanohybrids (NHs), in which many small metal nanoparticles are encapsulated within a conductive polymer matrix, are useful as sensitive electrochemical labels because the constituents produce characteristic oxidation current responses. Gold NHs, consisting of gold nanoparticles and poly­(m-toluidine), and copper NHs, consisting of copper nanoparticles and polyaniline, did not interfere with each other in terms of the electrochemical signals obtained on the same electrode. Antibodies were introduced into these NHs to function as electrochemical labels for targeting specific bacteria. Electrochemical measurements using screen-printed electrodes dry-fixed with NH-labeled bacterial cells enabled the estimation of bacterial species and number within minutes, based on the distinct current response of the labels. Our proposed method achieved simultaneous detection of enterohemorrhagic Escherichia coli and Staphylococcus aureus in a real sample. These NHs will be powerful tools as electrochemical labels and are expected to be useful for rapid testing in food and drug-related manufacturing sites.
doi_str_mv 10.1021/acs.analchem.3c04587
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects analytical chemistry
Antibodies
Bacteria
Biosensing Techniques - methods
Conducting polymers
Copper
copper nanoparticles
E coli
Electrochemical analysis
Electrochemical Techniques
Electrochemistry
Electrodes
enterohemorrhagic Escherichia coli
Gold
Gold - chemistry
Labels
Limit of Detection
Metal Nanoparticles - chemistry
nanogold
nanohybrids
Nanoparticles
Oxidation
Oxidation-Reduction
polyaniline
Polyanilines
Polymers
Staphylococcus aureus
Toluidine
title Simultaneous Electrochemical Detection of Multiple Bacterial Species Using Metal–Organic Nanohybrids
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