Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells

Pteropine orthoreovirus (PRV) causes respiratory tract infections in humans. Despite its emergence as a zoonotic and respiratory virus, little is known about its cell tropism, which hampers progress in fully understanding its pathogenesis in humans. Hek293 cells are most susceptible to PRV infection...

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Published in:Microbes and infection 2024-01, Vol.26 (1-2), p.105243, Article 105243
Main Authors: Siew, Zhen Yun, Tan, Yeh Fong, Iswara, Reuben Parama, Wong, Shew Fung, Wong, Siew Tung, Tan, Boon Keat, Leong, Pooi Pooi, Tan, Chee Wah, Wang, Lin-Fa, Leong, Chee Onn, Voon, Kenny
Format: Article
Language:English
Subjects:
Animals
Fish Diseases
HEK293 Cells
HeLa Cells
Humans
Keratins
Orthoreovirus
Reoviridae Infections - pathology
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Summary:Pteropine orthoreovirus (PRV) causes respiratory tract infections in humans. Despite its emergence as a zoonotic and respiratory virus, little is known about its cell tropism, which hampers progress in fully understanding its pathogenesis in humans. Hek293 cells are most susceptible to PRV infection, while HeLa cells are the least. Human cytokeratin 1 (CK1) was identified as the protein that interacts with PRV. The immunofluorescence assay and qPCR results revealed prior treatment with anti-CK1 may provide Hek293 cells protection against PRV. The KRT1-knockout Hek293 cells were less susceptible to PRV infection. Further study into the pathogenesis of PRV in humans is needed.
ISSN:1286-4579
1769-714X
1769-714X
DOI:10.1016/j.micinf.2023.105243