TCM formula for trauma treatment screening and its role of promoting infectious wound coalescence investigating

• Published in: Research in veterinary science 2024-04, Vol.170, p.105178-105178, Article 105178
• Main Authors: Li, Siya, Gu, Bolin, Meng, Jinwu, Zhu, Jinyue, Wang, Jinli, Wang, Weiran, Ding, Jinxue, Qiu, Tianxin, Wang, Wenjia, Liu, Jiaguo, Wu, Yi, Li, Kun
• Format: Article
• Language: English
• Subjects: Growth factor; Pet traumatic wound; Skin fibroblasts; TCM prescription; Wound healing
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2024.105178

In pet clinics, the number of cases using trauma drugs accounts for >10% of the total number of cases, and most wounds are healing by second intention. The prolongation of wound healing time causes inconvenience and burden to pets and pet owners. Therefore, how to reduce wound healing time and achieve maximum recovery of tissue function and aesthetics is one of the focuses of veterinary clinical practice. Wound suppuration caused by Staphylococcus aureus and Pseudomonas aeruginosa is the main cause of delaying wound healing. Clinically, available antimicrobial treatments are almost exhausted due to the production of large numbers of resistant bacteria. At present, there are no bacteria resistant to traditional Chinese medicine (TCM), which makes TCM have the potential to become an effective drug for the treatment of bacterial infections, so the use of TCM in the treatment of traumatic infections has broad prospects. Based on the characteristics of infection syndrome, three different prescriptions were formulated in our laboratory, and the most effective prescription and dosage form was screened and named Lianrong Healing Cream (LRHC). The results showed that LRHC regulated the expression of fibroblast growth factor-2 (FGF-2), epidermal growth factor-1 (EGF-1), transforming growth factor-β (TGF-β) and vascular endothelial growth factor-1 (VEGF-1) genes in wound tissues and fibroblasts, thereby accelerating wound healing and repairing wound appearance and function. The results of this study may be help to develop TCM formulation for traumatic infections. •Three TCM prescriptions for infectious trauma was developed. The optimal prescription was named Lianrong Healing Cream (LRHC).•Using pathogenic bacteria isolated from typical cases, a traumatic infection trauma model was successfully constructed.•LRHC can improve infectious trauma healing by regulating the expression of growth factors.•LRHC can affect the secretion of growth factors by fibroblasts.