Identifications of three novel alleles of Serrate in Drosophila

• Published in: Cells & development 2024-03, Vol.177, p.203908-203908, Article 203908
• Main Authors: Zhang, Qinghai, Zhang, Pei, Yang, Min, Tian, Yingxue, Feng, Chunxia, Wei, Wei
• Format: Article
• Language: English
• Subjects: Alleles; Animals; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Drosophila; Drosophila - genetics; Drosophila - metabolism; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Jagged-1 Protein - genetics; Membrane Proteins - genetics; Membrane Proteins - metabolism; Notch signaling pathway; Receptors, Notch - genetics; Receptors, Notch - metabolism; Serrate; Serrate-Jagged Proteins - genetics; Serrate-Jagged Proteins - metabolism; Wing development
• ISSN: 2667-2901; 2667-2901
• DOI: 10.1016/j.cdev.2024.203908

The Notch signaling pathway, an evolutionarily highly conserved pathway, participates in various essential physiological processes in organisms. Activation of Notch signaling in the canonical manner requires the combination of ligand and receptor. There are two ligands of Notch in Drosophila: Delta (Dl) and Serrate (Ser). A mutation mf157 is identified for causing nicks of fly wings in genetic analysis from a mutant library (unpublished) that was established previously. Immunofluorescent staining illustrates that mf157 represses the expression of Cut and Wingless (Wg), the targets of Notch signaling. MARCM cloning analysis reveals that mf157 functions at the same level or the upstream of ligands of Notch in signaling sending cells. Sequencing demonstrates that mf157 is a novel allele of the Ser gene. Subsequently, mf553 and mf167 are also identified as new alleles of Ser from our library. Furthermore, the complementary assays and the examination of transcripts confirm the sequencing results. Besides, the repressed phenotypes of Notch signaling were reverted by transposon excision experiments of mf157. In conclusion, we identify three fresh alleles of Ser. Our works supply additional genetic resources for further study of functions of Ser and Notch signaling regulation. •Three mutants with notched wing phenotype were identified by direct mosaic clone analysis.•These mutants were related with Notch signaling through detection of target gene and epistatic analysis.•They are novel alleles of serrate identified by inverse PCR.•There are 3 effective and different mutations for studying Serrate functions.