Nanoparticulate bioceramic putty suppresses osteoclastogenesis and inflammatory bone loss in mice via inhibition of TRAF6‐mediated signalling pathways: A laboratory investigation

Aim This study aimed to determine the effects of iRoot BP Plus on receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclastogenesis in vitro and inflammation‐mediated bone resorption in vivo and investigated the underlying molecular mechanisms. Methodology CCK‐8 was performed to test...

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Bibliographic Details
Published in:International endodontic journal 2024-06, Vol.57 (6), p.682-699
Main Authors: Wang, Zijun, Zhang, Jie, Sun, Xiaoyue, Yu, Jingjing, Liu, Bingqian, Peng, Bin, Wang, Li, Yang, Jingwen, Zhu, Lingxin
Format: Article
Language:English
Subjects:
bioceramic
Bone loss
Bone resorption
Bone turnover
Calvaria
Cathepsin K
Cell viability
Cholecystokinin
Gene expression
Homeostasis
Inflammation
Kinases
lipopolysaccharide
Molecular modelling
mouse calvarial osteolysis model
Nanoparticles
Nuclear transport
osteoclast
Osteoclastogenesis
Osteolysis
Phalloidin
Phosphorylation
Proteasomes
Signal transduction
TRAF6
TRAF6 protein
TRANCE protein
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Summary:Aim This study aimed to determine the effects of iRoot BP Plus on receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclastogenesis in vitro and inflammation‐mediated bone resorption in vivo and investigated the underlying molecular mechanisms. Methodology CCK‐8 was performed to test cell viability in RANKL‐induced RAW 264.7 cells and BMDMs in response to iRoot BP Plus. The effect of iRoot BP Plus on osteoclastogenesis was determined using TRAP staining and phalloidin staining, respectively. Pit formation assay was conducted to measure osteoclast resorptive capacity. Western blot and qPCR were performed to examine osteoclast‐related proteins and gene expression, respectively. Western blot was also used to investigate the signalling pathways involved. For in vivo experiments, an LPS‐induced mouse calvarial bone resorption model was established to analyse the effect of iRoot BP Plus on bone resorption (n = 6 per group). At 7 days, mouse calvaria were collected and prepared for histological analysis. Results We identified that iRoot BP Plus extracts significantly attenuated RANKL‐induced osteoclastogenesis, reduced sealing zone formation, restrained osteolytic capacity and decreased osteoclast‐specific gene expression (p 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.14051