Rickettsia species identified in adult, host-seeking Dermacentor occidentalis (Acari: Ixodidae) from Baja California, Mexico, and Oregon and Washington, United States

• Published in: Journal of medical entomology 2024-05, Vol.61 (3), p.781-790
• Main Authors: Paddock, Christopher D., Zambrano, Maria L., Clover, James R., Ladd-Wilson, Stephen, Dykstra, Elizabeth A., Salamone, Amy, Kangiser, David, Ayres, Bryan N., Shooter, Savannah L., Karpathy, Sandor E., Kjemtrup, Anne M., Beati, Lorenza, Levin, Michael L., Lane, Robert S., Zazueta, Oscar E.
• Format: Article
• Language: English
• Subjects: Adults; Arachnids; Deoxyribonucleic acid; Dermacentor occidentalis; Disease control; DNA; Entomology; Fever; Gene sequencing; Genotypes; Introduced species; Knowledge representation; Nucleotide sequence; Pacific Coast tick; Pathogens; Populations; Rickettsia; Ticks; VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION
• ISSN: 0022-2585; 1938-2928; 1938-2928
• DOI: 10.1093/jme/tjae023

The Pacific Coast tick (Dermacentor occidentalis Marx, 1892) is a frequently encountered and commonly reported human-biting tick species that has been recorded from most of California and parts of southwestern Oregon, southcentral Washington, and northwestern Mexico. Although previous investigators have surveyed populations of D. occidentalis for the presence of Rickettsia species across several regions of California, populations of this tick have not been surveyed heretofore for rickettsiae from Baja California, Oregon, or Washington. We evaluated 1,367 host-seeking, D. occidentalis adults collected from 2015 to 2022 by flagging vegetation at multiple sites in Baja California, Mexico, and Oregon and Washington, United States, using genus- and species-specific assays for spotted fever group rickettsiae. DNA of Rickettsia 364D, R. bellii, and R. tillamookensis was not detected in specimens from these regions. DNA of R. rhipicephali was detected in D. occidentalis specimens obtained from Ensenada Municipality in Baja California and southwestern Oregon, but not from Washington. All ompA sequences of R. rhipichephali that were amplified from individual ticks in southwestern Oregon were represented by a single genotype. DNA of the Ixodes pacificus rickettsial endosymbiont was amplified from specimens collected in southwestern Oregon and Klickitat County, Washington; to the best of our knowledge, this Rickettsia species has never been identified in D. occidentalis. Collectively, these data are consistent with a relatively recent introduction of Pacific Coast ticks in the northernmost extension of its recognized range.