Dermal thirdhand smoke exposure induced epidermal alterations in human keratinocyte cells through oxidative damage and MMP‐1 expression

Thirdhand smoke (THS) is the residual cigarette smoke that settles on indoor surface fabrics, dust and can accumulate in the environment. Therefore, it can be a risk factor for individuals who have frequent dermal contact with THS‐contaminated surfaces. In the present study, it was aimed to elucidat...

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Bibliographic Details
Published in:Experimental dermatology 2024-02, Vol.33 (2), p.e15020-n/a
Main Authors: Reis, Rengin, Kolci, Kubra, Yedikardes, Eda Nil, Coskun, Goknil Pelin, Uzuner, Yasemin
Format: Article
Language:English
Subjects:
Cigarette smoke
Cytotoxicity
Inflammation
Intracellular
Keratinocytes
Matrix metalloproteinase
Metalloproteinase
oxidative stress
Risk factors
Skin diseases
Sulforaphane
Superoxide dismutase
thirdhand smoke
Wound healing
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Summary:Thirdhand smoke (THS) is the residual cigarette smoke that settles on indoor surface fabrics, dust and can accumulate in the environment. Therefore, it can be a risk factor for individuals who have frequent dermal contact with THS‐contaminated surfaces. In the present study, it was aimed to elucidate the toxicity of dermal THS exposure in HaCaT human keratinocytes. The THS was extracted from terrycloth exposed to 3R4F research cigarette smoke in a closed chamber and the adverse outcomes induced by THS were determined through assessment of cytotoxicity tests (MTT and NRU), intracellular GSH level, total SOD activity, matrix metalloproteinase‐1 (MMP‐1) and IL‐6 levels. The wound healing capacity of THS‐exposed keratinocytes was evaluated via scratch assay. A potent antioxidant isothiocyanate compound, sulforaphane (SFN), was used as a negative control. THS was dose‐dependently cytotoxic (12.5%–100%, v/v) to the HaCaT cells through mitochondrial cell dysfunction (p 
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.15020