Enhanced Microbial Protein Production from CO2 and Air by a MoS2 Catalyzed Bioelectrochemical System

• Published in: ChemPlusChem (Weinheim, Germany) Germany), 2024-08, Vol.89 (8), p.e202400072-n/a
• Main Authors: Zhang, Lixia, Zeng, Lizhen, Wang, Jingting, Wang, Haoran, Zheng, Decong, Wang, Xiaomei, Li, Daping, Zhan, Guoqiang
• Format: Article
• Language: English
• Subjects: Bioelectrochemical system; CO2 reduction; Microbial protein; MoS2 electrocatalyst; N2 reduction
• ISSN: 2192-6506; 2192-6506
• DOI: 10.1002/cplu.202400072

Carbon dioxide can be relatively easily reduced to organic matter in a bioelectrochemical system (BES). However, due to insufficient reduction force from in‐situ hydrogen evolution, it is difficult for nitrogen reduction. In this study, MoS2 was firstly used as an electrocatalyst for the simultaneous reduction of CO2 and N2 to produce microbial protein (MP) in a BES. Cell dry weight (CDW) could reach 0.81±0.04 g/L after 14 d operation at −0.7 V (vs. RHE), which was 108±3 % higher than that from non‐catalyst control group (0.39±0.01 g/L). The produced protein had a better amino acid profile in the BES than that in a direct hydrogen system (DHS), particularly for proline (Pro). Besides, MoS2 promoted the growth of bacterial cell on an electrode and improved the biofilm extracellular electron transfer (EET) by microscopic observation and electrochemical characterization of MoS2 biocathode. The composition of the microbial community and the relative abundance of functional enzymes revealed that MoS2 as an electrocatalyst was beneficial for enriching Xanthobacter and enhancing CO2 and N2 reduction by electrical energy. These results demonstrated that an efficient strategy to improve MP production of BES is to use MoS2 as an electrocatalyst to shift amino acid profile and microbial community. A MoS2 catalyzed bioelectrochemical system is successfully constructed, in which microbial protein can be produced from the simplest small molecule substances (CO2, N2, and H2O) and Xanthobacter‐dominanted hydrogen oxidizing bacteria can be enriched. Additionally, MoS2 can improve biofilm extracellular electron transfer (EET) and enhance proline production.