Talaromyces sedimenticola sp. nov., isolated from the Mariana Trench

• Published in: Antonie van Leeuwenhoek 2024-12, Vol.117 (1), p.44, Article 44
• Main Authors: Zhou, Hongbo, Xu, Liting, Liu, Wenxian, Ta, Kaiwen, Wang, Xincun, Guo, Jianwei, Luo, Wenxi, Peng, Zhiyuan, Huang, Qiaoni, Wang, Yuguang
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Biomedical and Life Sciences; Calmodulin; DNA, Bacterial - genetics; DNA-directed RNA polymerase; Fatty Acids - chemistry; Gene sequencing; Life Sciences; Medical Microbiology; Microbiology; Morphology; New species; Original Paper; Phylogenetics; Phylogeny; Physical characteristics; Physicochemical properties; Plant Sciences; RNA polymerase; RNA polymerase II; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Science & Conservation; Talaromyces; Tubulin
• ISSN: 0003-6072; 1572-9699; 1572-9699
• DOI: 10.1007/s10482-024-01945-1

Two fungal strains (K-2 T and S1) were isolated from the deepest ocean sediment of the Challenger Deep located in the Mariana Trench. The internal transcribed spacer (ITS) gene sequences of the isolates K-2 T and S1 differed from those of closely related species, such as Talaromyces assiutensis and T. trachyspermus . Phylogenetic analyses based on single and concatenated alignments of the genes, namely ITS, β -tubulin ( benA ), calmodulin ( cam ), and the second-largest subunit fragment of the RNA polymerase II ( rpb2 ) showed that the isolates K-2 T and S1 were clustered together with other Talaromyces species , such as T. trachyspermus and T. assiutensis , as evidenced by the position on a terminal branch with high bootstrap support. They could also be distinguished from their closest relatives with valid published names via morphological and physiological characteristics, for example, growth at 4 °C–50 °C with a pH in the range of 1.5–12. Based on their phylogenetic, morphological, and physicochemical properties, the isolates K-2 T and S1 represent a novel species in the genus Talaromyces , and the proposed name is Talaromyces sedimenticola sp. nov. The type strain is K-2 T (= GDMCC 3.746 T  = JCM 39451 T ).