Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs

Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STR...

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Bibliographic Details
Published in:International journal of legal medicine 2024-07, Vol.138 (4), p.1255-1264
Main Authors: Guo, Li-Liang, Yuan, Jia-Hui, Zhang, Chi, Zhao, Jie, Yao, Yi-Ren, Guo, Ke-Li, Meng, Yang, Ji, An-Quan, Kang, Ke-Lai, Wang, Le
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Deoxyribonucleic acid
DNA
Electrophoresis
Forensic Medicine
Forensic sciences
Genetic testing
Intergenerational relationships
Invoices
Legal medicine
Medical Law
Medicine
Medicine & Public Health
Original Article
Sex crimes
Standard reference materials
Citations: Items that this one cites
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Summary:Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STRs, and the Amelogenin locus. Amplicon sizes of 117 loci were below 300 bp. In this study, MiSeq FGx sequencing metrics for STRseqTyper122 were presented. The genotyping accuracy of this kit was examined by comparing to certified genotypes of NIST standard reference materials and results from five capillary electrophoresis-based kits. The sensitivity of STRseqTyper122 reached 125 pg, and > 80% of the loci were correctly called with 62.5 pg and 31.25 pg input genomic DNA. Repeatability, species specificity, and tolerance for DNA degradation and PCR inhibitors of this kit were also evaluated. STRseqTyper122 demonstrated reliable performance with routine case-work samples and provided a powerful tool for forensic applications.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-024-03195-2