ITGB6 promotes pancreatic fibrosis and aggravates the malignant process of pancreatic cancer via JAK2/STAT3 signaling pathway

• Published in: Naunyn-Schmiedeberg's archives of pharmacology 2024-08, Vol.397 (8), p.6093-6106
• Main Authors: Zhang, Yu, Chen, Zhiyuan, Shen, Zhengchao, Qian, Daohai, Wang, Guannan, Wang, Xu, Xi, Shihang, Wang, Xiaoming
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell activation; Cell culture; Cell Line, Tumor; Cell migration; Cell Movement; Cell Proliferation; Cell suspensions; Cholecystokinin; Fibrosis; Flow cytometry; Humans; Immunofluorescence; Integrin beta Chains - genetics; Integrin beta Chains - metabolism; Janus kinase 2; Janus Kinase 2 - metabolism; Male; Malignancy; Metastases; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Invasiveness; Neurosciences; Pancreas - metabolism; Pancreas - pathology; Pancreatic cancer; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pancreatic Stellate Cells - metabolism; Pancreatic Stellate Cells - pathology; Pancreatitis; Pharmacology/Toxicology; Signal Transduction; Stat3 protein; STAT3 Transcription Factor - metabolism; Stellate cells; Transforming growth factor-b; Western blotting; Wound healing
• ISSN: 0028-1298; 1432-1912; 1432-1912
• DOI: 10.1007/s00210-024-03003-z

Integrin β6 (ITGB6) is upregulated in multiple tumor types and elevated ITGB6 levels have been detected in patients with chronic pancreatitis. However, the role of ITGB6 in pancreatic fibrosis and cancer remains to be elucidated. In the present study, ITGB6 expression was assessed using western blotting and qRT-PCR. Besides, cell proliferation, cycling, migration, and invasion were evaluated using CCK-8, flow cytometry, wound healing, and transwell assays, respectively. The expression of fibrosis and JAK2/STAT3 signaling markers was detected by western blotting and immunofluorescence analysis. Moreover, nude mice were subcutaneously injected with co-cultured cell suspensions to establish an in vivo model. The results showed that ITGB6 was highly expressed in pancreatic cancer tissues and TGF-β-induced pancreatic stellate cells (PSCs). Inhibition of ITGB6 expression in PSCs resulted in clear inhibition of activated PSC proliferation, migration, and fibrogenesis. Additionally, reduced ITGB6 expression inhibits the JAK2/STAT3 signaling pathway. Interestingly, activators of the JAK2/STAT3 signaling pathway reversed the effects of ITGB6 disruption on PSCs. Activated PSCs notably promoted the proliferation, invasion, and migration of pancreatic cancer cells in a co-culture assay. In contrast, activated PSCs with low ITGB6 expression failed to significantly affect the malignancy of pancreatic cancer cells. Moreover, in vivo results showed that interference with ITGB6 inhibited the activation of PSCs and promoted the development of pancreatic cancer. Silencing ITGB6 inhibited the proliferation, migration, and fibrosis-like effects of activated PSCs and indirectly inhibited the metastasis and malignant process of pancreatic cancer by inhibiting the JAK2/STAT3 signaling pathway. Therefore, ITGB6 is a potential candidate target for pancreatic cancer prevention and treatment.