A method for studying escape behavior to terrestrial threats in rodents

• Published in: Journal of neuroscience methods 2024-05, Vol.405, p.110099, Article 110099
• Main Authors: Zhang, Yueting, Wang, Jincheng, Pang, Ruiqi, Zhang, Yanjie, Deng, Qiyue, Liu, Xue, Zhou, Yi
• Format: Article
• Language: English
• Subjects: Animals; Behavior, Animal; Closed-loop interactive platform; Escape behaviour; Escape Reaction - physiology; Mice; Predatory Behavior - physiology; Rodentia; Terrestrial threats
• ISSN: 0165-0270; 1872-678X; 1872-678X
• DOI: 10.1016/j.jneumeth.2024.110099

Escape is one of the most essential behaviors for an animal's survival because it could be a matter of life and death. Much of our current understanding of the neural mechanisms underlying escape is derived from the looming paradigm, which mimics a diving aerial predator. Yet, the idea of the looming paradigm does not account for all types of threats like lions hunting antelopes or cats stalking mice. Escape responses to such terrestrial threats may require different strategies and neural mechanisms. Here, we developed a real-time interactive platform to study escape behavior to terrestrial threats in mice. A closed-loop controlled robot was magnetically pulled to mimic a terrestrial threat that chases a mouse. By using strong magnets and high-precision servo motors, the robot is capable of moving precisely with a high spatial-temporal resolution. Different algorithms can be used to achieve single approach or persistent approach. Animal experiments showed that mice exhibited consistent escape behavior when exposed to an approaching robotic predator. When presented with a persistently approaching predator, the mice were able to rapidly adapt their behavior, as evidenced by a decrease in startle responses and changes in movement patterns. In comparison to existing methods for studying escape behavior, such as the looming paradigm, this approach is more suitable for investigating animal behavior in response to sustained threats. In conclusion, we have developed a flexible platform to study escape behavior to terrestrial threats in mice. •A flexible platform to study escape behavior to terrestrial threats in mice.•Mice perceive the approaching robotic predator as a threat and escape quickly.•Mice can quickly adapt their behavior when facing a persistent threat.•The visual information is still critical in eliciting escape behavior to terrestrial threats.