Drought responses and population differentiation of Calohypnum plumiforme inferred from comparative transcriptome analysis

• Published in: Plant physiology and biochemistry 2024-03, Vol.208, p.108456-108456, Article 108456
• Main Authors: Xing, Chengguang, Lei, Chunyi, Yang, Yuchen, Zhou, Dandan, Liu, Shanshan, Xu, Jianqu, Liu, Zhiwei, Wu, Tao, Zhou, Xiaohang, Huang, Shuzhen, Liu, Weiqiu
• Format: Article
• Language: English
• Subjects: Bryophytes; Desiccation-tolerance; Gene regulatory network; Local adaptation; Phytohormones; Population differentiation; RNA-Seq
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108456

Bryophytes, known as poikilohydric plants, possess vegetative desiccation-tolerant (DT) ability to withstand water deficit stress. Consequently, they offer valuable genetic resources for enhancing resistance to water scarcity stress. In this research, we examined the physiological, phytohormonal, and transcriptomic changes in DT mosses Calohypnum plumiforme from two populations, with and without desiccation treatment. Comparative analysis revealed population differentiation at physiological, gene sequence, and expression levels. Under desiccation stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) showed significant increases, along with elevation of soluble sugars and proteins, consistent with the transcriptome changes. Notable activation of the bypass pathway of JA biosynthesis suggested their roles in compensating for JA accumulation. Furthermore, our analysis revealed significant correlations among phytohormones and DEGs in their respective signaling pathway, indicating potential complex interplays of hormones in C plumiforme. Protein phosphatase 2C (PP2C) in the abscisic acid signaling pathway emerged as the pivotal hub in the phytohormone crosstalk regulation network. Overall, this study was one of the first comprehensive transcriptome analyses of moss C. plumiforme under slow desiccation rates, expanding our knowledge of bryophyte transcriptomes and shedding light on the gene regulatory network involved in response to desiccation, as well as the evolutionary processes of local adaptation across moss populations. •Desiccation stress induces phytohormone accumulation and antioxidant enzyme activity.•Transcriptomic analysis reveals population differentiation in C. plumiforme.•DEGs highlight the role of POD, SOD, Glucose, and LEAs in stress response.•PP2Cs act as central regulators in phytohormone crosstalk.