Annual recurrence of prokaryotic climax communities in shallow waters of the North Mediterranean

• Published in: Environmental microbiology 2024-03, Vol.26 (3), p.e16595-n/a
• Main Authors: Celussi, Mauro, Manna, Vincenzo, Banchi, Elisa, Fonti, Viviana, Bazzaro, Matteo, Flander‐Putrle, Vesna, Klun, Katja, Kralj, Martina, Orel, Neža, Tinta, Tinkara
• Format: Article
• Language: English
• Subjects: Abiotic factors; Archaea - genetics; Climax communities; Coastal environments; Coastal zones; Ecosystem; Heterotrophic organisms; High temperature; Intercomparison; Microbial activity; Microbiomes; Microorganisms; Organic matter; Photoperiods; Phytoplankton; Phytoplankton - genetics; Plankton - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater - chemistry; Sequences; Shallow water; Substrates; Time series; Typology
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.16595

In temperate coastal environments, wide fluctuations of biotic and abiotic factors drive microbiome dynamics. To link recurrent ecological patterns with planktonic microbial communities, we analysed a monthly‐sampled 3‐year time series of 16S rRNA amplicon sequencing data, alongside environmental variables, collected at two stations in the northern Adriatic Sea. Time series multivariate analyses allowed us to identify three stable, mature communities (climaxes), whose recurrence was mainly driven by changes in photoperiod and temperature. Mixotrophs (e.g., Ca. Nitrosopumilus, SUP05 clade, and Marine Group II) thrived under oligotrophic, low‐light conditions, whereas copiotrophs (e.g., NS4 and NS5 clades) bloomed at higher temperatures and substrate availability. The early spring climax was characterised by a more diverse set of amplicon sequence variants, including copiotrophs associated with phytoplankton‐derived organic matter degradation, and photo‐auto/heterotrophic organisms (e.g., Synechococcus sp., Roseobacter clade), whose rhythmicity was linked to photoperiod lengthening. Through the identification of recurrent climax assemblages, we begin to delineate a typology of ecosystem based on microbiome composition and functionality, allowing for the intercomparison of microbial assemblages among different biomes, a still underachieved goal in the omics era. Recurrent environmental patterns structure Mediterranean coastal microbial communities in climactic assemblages. Copiotrophic taxa (Rhodobacteraceae, NS4, NS5) associated with phytoplankton‐derived organic matter recur in spring; mixotrophs (Nitrosopumilus, SUP05, MGII) characterise vernal climax; summertime oligotrophic conditions favour Synechococcus domination among photoautotrophs and oligotypes exploiting organic matter.