Pepper defense against Ralstonia solanacearum and High-temperature stress is positively regulated by CaMYB59

• Published in: Microbial pathogenesis 2024-04, Vol.189, p.106599-106599, Article 106599
• Main Authors: Noman, Ali, Alwutayd, Khairiah Mubarak, Aqeel, Muhammad, Hussain, Ansar, Qasim, Muhammad, Al-Qthanin, Rahmah N., Alshaharni, Mohammed O., Alzuaibr, Fahad Mohammed, Alomran, Maryam M.
• Format: Article
• Language: English
• Subjects: Disease Resistance - genetics; Genes; Humans; Hydrogen Peroxide - metabolism; Pathogen; Plant Diseases - genetics; Plant Growth Regulators - genetics; Plant Immunity - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Ralstonia solanacearum - genetics; RNA; Survival; Temperature
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2024.106599

We have functionally evaluated a transcription factor CaMYB59 for its role in pepper immune responses to Ralstonia solanacearum attack and high temperature-high humidity (HTHH). Exposure to R. solanacearum inoculation (RSI) and HTHH resulted in up-regulation of this nucleus-localized TF. Function of this TF was confirmed by performing loss of function assay of CaMYB59 by VIGS (virus-induced gene silencing). Plants with silenced CaMYB59 displayed not only compromised pepper immunity against RSI but also impaired tolerance to HTHH along with decreased hypersensitive response (HR). This impairment in defense function was fully linked with low induction of stress-linked genes like CaPO2, CaPR1, CaAcc and thermo-tolerance linked CaHSP24 as well as CaHsfB2a. Conversely, transient overexpression of CaMYB59 enhanced pepper immunity. This reveals that CaMYB59 positively regulated host defense against RSI and HTHH by means of HR like mimic cell death, H2O2 production and up-regulation of defense as well as thermo-tolerance associated genes. These changes in attributes collectively confirm the role of CaMYB59 as a positive regulator of pepper immunity against R. solanacearum. We recommend that such positive regulation of pepper defense is dynamically supported by phyto-hormone signaling and transcriptional web of defense genes. These integrated and interlinked events stabilize plant growth and survival under abiotic and biotic stresses. •Plants identify microbial pathogens and trigger their defense.•CaMYB59 is induced by the RSI and HTHH and activates HR mimic response.•Silencing of CaMYB59 impaired pepper immunity and thermo-tolerance.•This acts as a beneficial regulator in pepper immunity.