Soybean RVE8a confers salt and drought tolerance in Arabidopsis

• Published in: Biochemical and biophysical research communications 2024-04, Vol.704, p.149660, Article 149660
• Main Authors: Bao, Guohua, Sun, Guoqing, Wang, Jingying, Shi, Tianran, Xu, Xiao, Zhai, Lulu, Bian, Shaomin, Li, Xuyan
• Format: Article
• Language: English
• Subjects: Circadian; Drought and salt stress; GmNAC17; GmRVE8a; Soybean
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2024.149660

Soybean is an economically important crop, which often suffers various abiotic stresses. REVEILLE (RVE) genes have been generally considered as circadian oscillators to mediate diverse developmental processes and plant response to environmental stresses. Addressing their roles is of significance for utilizing them to enhance agronomic traits in crops. However, our understanding of soybean RVEs is extremely limited. In the study, we investigated the expression patterns of soybean CCA1-like genes under salt stress using our RNA-Seq data. Subsequently, a salt stress-inducible gene, GmRVE8a, was chosen for further study. Phylogenetic analysis indicated that GmRVE8a is most closely related to Arabidopsis RVE4 and RVE8. Also, GmRVE8a showed circadian expression pattern with 24 h rhythmic period, suggesting that it might be a clock-regulated gene. Moreover, transgenic Arabidopsis lines over-expressing GmRVE8a were generated. It was observed that ectopic over-expression of GmRVE8a caused a significant delay in flowering. Further observation indicated that under salt and drought stress, transgenic seedlings were stronger than wild type. Consistently, three-week-old transgenic plants grew better than wild type under salt and drought conditions, and the MDA content in transgenic lines was significantly lower than wild type, suggesting that GmRVE8a might be a positive regulator in response to salt and drought stress. Intriguingly, Y2H assay indicated that GmRVE8a physically interacted with a drought-tolerant protein, GmNAC17. Overall, our findings provided preliminary information regarding the functional roles of GmRVE8a in response to salt and drought stress. •GmRVE8a is most closely related to the Arabidopsis RVE4 and RVE8.•GmRVE8a showed abiotic stress-inducible and rhythmic expression patterns.•Over-expression of GmRVE8a in Arabidopsis improved plant tolerance to drought and salt stress.