Combined effect of Neurotropin® and methylcobalamin on postherpetic neuralgia in mice infected with herpes simplex virus type-1

• Published in: Journal of dermatological science 2024-03, Vol.113 (3), p.138-147
• Main Authors: Andoh, Tsugunobu, Kikukawa, Takashi, Kotani, Atsushi, Kurokawa, Yoko, Asakura, Wakana, Houmoto, Kengo, Fukutomi, Daisuke, Uta, Daisuke, Okai, Hisashi, Koike, Koji
• Format: Article
• Language: English
• Subjects: herpes simplex virus; methylcobalamin; neurotropin; postherpetic neuralgia; primary sensory neuron
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2024.02.004

Postherpetic pain (PHP) is difficult to control. Although Neurotropin® (NTP) and methylcobalamin (MCB) are often prescribed to treat the pain, the efficacy of combined treatment for PHP remains imcompletely understood. In this study, we investigate the combined effects of NTP and MCB on PHP in mice. NTP and MCB were administered from day 10–29 after herpes simplex virus type-1 (HSV-1) infection. The pain-related responses were evaluated using a paint brush. The expression of neuropathy-related factor (ATF3) and nerve repair factors (GAP-43 and SPRR1A) in the dorsal root ganglion (DRG) and neurons in the skin were evaluated by immunohistochemical staining. Nerve growth factor (NGF) and neurotrophin-3 (NT3) mRNA expression levels were evaluated using real-time PCR. Repeated treatment with NTP and MCB after the acute phase inhibited PHP. Combined treatment with these drugs inhibited PHP at an earlier stage than either treatment alone. In the DRG of HSV-1-infected mice, MCB, but not NTP, decreased the number of cells expressing ATF3 and increased the number of cells expressing GAP-43- and SPRR1A. In addition, MCB, but not NTP, also increased and recovered non-myelinated neurons decreased in the lesional skin. NTP increased the mRNA levels of NTF3 in keratinocytes, while MCB increased that of NGF in Schwann cells. These results suggest that combined treatment with NTP and MCB is useful for the treatment of PHP. The combined effect may be attributed to the different analgesic mechanisms of these drugs. •Neurotropin® and methylcobalamin to postherpetic pain (PHP) were evaluated in mice.•Combined treatment with these drugs to PHP showed faster efficacy than either drug.•Methylcobalamin effected the expression of the factors on neuropathy or nerve repair.•These drugs induced the gene expression on neurotropic factors.•Combined treatment with these drugs is more effective to PHP than each drug alone.