Effects of litter amount and seed sowing position on seedling emergence and growth of hemiparasitic Rhinanthus species under drought stress

• Published in: Plant biology (Stuttgart, Germany) Germany), 2024-04, Vol.26 (3), p.437-445
• Main Authors: Zimmerbeutel, A., Diekötter, T., Reck, H., Nissen, H., Wiedmann, L. J., Donath, T. W.
• Format: Article
• Language: English
• Subjects: Biomass; biomass production; Drought; Droughts; Emergence; Environmental impact; Flowers & plants; Germination; Grass growth; Grasses; Grasslands; Litter; litter layer; Orobanchaceae; Plant species; Planting; Plants; Plants (botany); Poaceae; Rhinanthus; Rhinanthus angustifolius; Rhinanthus minor; roadside vegetation; Roadsides; seed germination; Seedlings; Seeds; Soil surfaces; Species; Traffic Infrastructure Habitats (TIH); Vegetation; Water; Water availability; water level; Water levels
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.13633

Roadside vegetation in Central Europe is mostly species‐poor and dominated by a few grass species. Hemiparasitic plant species, including Rhinanthus spp., might effectively restrict grass growth, thereby making space for light‐dependent herb species. Despite the significance of abiotic site conditions for plant establishment in general, their effects on Rhinanthus establishment are less well known. We investigated combined effects of water availability, litter amount and seed position within litter on Rhinanthus seedling emergence and growth. Two parallel greenhouse experiments were conducted with R. angustifolius and R. minor. In these, we tested the impact of 200 or 400 g litter·m−2 with seeds sown beneath or on top of a litter layer under constantly humid or intermittently dry conditions on seedling emergence and biomass production of Rhinanthus. Presence of litter positively affected Rhinanthus seedling emergence when sown beneath the litter layer and reduced negative effects of water deficiency. Sowing beneath a litter layer increased seedling emergence by 157%, with similar effects at 200 and 400 g litter·m−2. Water level did not affect biomass production. Compared to R. minor, R. angustifolius had higher mean biomass, and its seedlings emerged earlier and in higher numbers. Our results indicate that Rhinanthus spp. react similarly to litter as non‐hemiparasitic plant species from temperate grasslands. Litter presence positively influenced Rhinanthus seedling emergence and growth under intermittently dry conditions. Its hemiparasitic characteristics might reduce drought impacts on biomass production. To ensure seed contact with the soil surface, seeds should be sown when no litter is present, or mulching should occur post‐sowing. Presence of litter was found to be favourable for seedling emergence and growth of Rhinanthus spp., especially under intermittently dry conditions, but to ensure seed contact with the soil surface, seeds should be sown in the absence of litter, or mulching should occur post‐sowing.