Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection

Glanders, caused by Burkholderia mallei, is a zoonotic disease of equids. Serologic testing for glanders is required by disease-free countries before international movement of equids. The World Organisation for Animal Health Terrestrial Manual recommends the complement fixation test (CFT) for cleara...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2024-03, Vol.36 (2), p.283-286
Main Authors: Grause, Juanita F., Elschner, Mandy C., Ledesma, Nicholas A., Murphy, Gleeson
Format: Article
Language:English
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Summary:Glanders, caused by Burkholderia mallei, is a zoonotic disease of equids. Serologic testing for glanders is required by disease-free countries before international movement of equids. The World Organisation for Animal Health Terrestrial Manual recommends the complement fixation test (CFT) for clearance of individual animals for movement, but the CFT is prone to false-positive results. A colorimetric western blot (WB) assay was developed and validated to resolve false-positive CFT results; however, that assay is relatively time-consuming, and the interpretation is subjective. We present here a procedurally similar chemiluminescent WB assay that performs comparably to the validated colorimetric WB assay and offers noticeable benefits of decreased time-to-result and greater ease of interpretation.
ISSN:1040-6387
1943-4936
DOI:10.1177/10406387241230292