Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed

Summary Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional syst...

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Bibliographic Details
Published in:Plant biotechnology journal 2006-01, Vol.4 (1), p.53-62
Main Authors: Clough, Richard C., Pappu, Kameshwari, Thompson, Kevin, Beifuss, Katherine, Lane, Jeff, Delaney, Donna E., Harkey, Robin, Drees, Carol, Howard, John A., Hood, Elizabeth E.
Format: Article
Language:English
Subjects:
bio-remediation
Biodegradation, Environmental
Biotechnology
Cell Wall - enzymology
Cytoplasm - enzymology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression
Genes, Fungal
Lignin - metabolism
ligninase
manganese peroxidase
Peroxidases - genetics
Peroxidases - metabolism
Phanerochaete - enzymology
Phanerochaete chrysosporium
pulp bleaching
Seeds - chemistry
Seeds - genetics
Seeds - metabolism
Transformation, Genetic
transgenic corn
Zea mays
Zea mays - chemistry
Zea mays - genetics
Zea mays - metabolism
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Summary:Summary Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large‐scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood‐rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full‐length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed‐preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high‐level expression, indicating genetic stability of the transgene.
ISSN:1467-7644
1467-7652
DOI:10.1111/j.1467-7652.2005.00157.x