Effect of high-frequency repetitive transcranial magnetic stimulation on swallowing function and pneumonia in poststroke dysphagia in rats

• Published in: Brain research 2024-06, Vol.1832, p.148846-148846, Article 148846
• Main Authors: Chen, Jiemei, Zhao, Fei, Hong, Jiena, Li, Chao, Zhang, Jiantao, Shan, Yilong, Ye, Qiuping, Wen, Hongmei
• Format: Article
• Language: English
• Subjects: Dysphagia; High-frequency repetitive transcranial magnetic stimulation; Pneumonia; Stroke; Videofluoroscopic swallowing study
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2024.148846

[Display omitted] •Compared with 5 Hz and 20 Hz HF-rTMS, 10 Hz HF-rTMS more effectively improved the swallowing function of rats with PSD.•HF-rTMS alleviated pneumonia in PSD rats.•HF-rTMS increased levels of SP in the lung, serum and NTS, increased levels of CGRP in the serum and NTS, increased levels of 5-HT in the NTS. Post-stroke dysphagia (PSD) is a common symptom of stroke. Clinical complications of PSD include malnutrition and pneumonia. Clinical studies have shown that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) can improve the swallowing function in stroke patients. However, few studies have elucidated the underlying molecular mechanisms. A PSD rat model was established using transient middle cerebral artery occlusion (tMCAO). Rats were randomly divided into sham-operated groups, PSD groups, PSD + sham-rTMS groups, PSD + 5 Hz-rTMS groups, PSD + 10 Hz-rTMS groups and PSD + 20 Hz-rTMS groups. Rats were weighed and videofluoroscopic swallowing studies were conducted. Pulmonary inflammation, levels of substance P (SP) and calcitonin gene-related peptide (CGRP) in the serum, lung, and nucleus tractus solitarius (NTS), brain-derived neurotrophic factor (BDNF) and 5-hydroxytryptamine (5HT) in NTS were evaluated. Rats in the PSD group experienced weight loss, reduced bolus area and pharyngeal bolus speed, and increased pharyngeal transit time (PTT) and inter-swallow interval (ISI) on day 7 and day 14 after operation. Moreover, PSD rats showed pulmonary inflammation, reduced levels of SP in the lung and serum, increased levels of CGRP in the lung and NTS, reduced levels of BDNF and 5HT in the NTS. There was no significant difference between the PSD group and the PSD + sham-rTMS group in the results of weight and VFSS. Comparing with the PSD group, there significant increases in the bolus area, decreases in PTT of rats following 5 Hz rTMS intervention. HF-rTMS at 10 Hz significantly increased the weight, bolus area, pharyngeal bolus speed and decreased the PTT and ISI of rats. There were also significant increases in the bolus area (p