The buccal micronucleus cytome assay: New horizons for its implementation in human studies

In this report we provide a summary of the presentations and discussion of the latest knowledge regarding the buccal micronucleus (MN) cytome assay. This information was presented at the HUMN workshop held in Malaga, Spain, in connection with the 2023 European, Environmental Mutagenesis and Genomics...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2024-02, Vol.894, p.503724-503724, Article 503724
Main Authors: Fenech, Michael, Knasmueller, Siegfried, Nersesyan, Armen, Bolognesi, Claudia, Wultsch, Georg, Schunck, Christian, Volpi, Emanuela, Bonassi, Stefano
Format: Article
Language:English
Subjects:
Automation
Buccal micronucleus
Clinical
DNA damage
Nutritional
Occupational
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Summary:In this report we provide a summary of the presentations and discussion of the latest knowledge regarding the buccal micronucleus (MN) cytome assay. This information was presented at the HUMN workshop held in Malaga, Spain, in connection with the 2023 European, Environmental Mutagenesis and Genomics conference. The presentations covered the most salient topics relevant to the buccal MN cytome assay including (i) the biology of the buccal mucosa, (ii) its application in human studies relating to DNA damage caused by environmental exposure to genotoxins, (iii) the association of buccal MN with cancer and a wide range of reproductive, metabolic, immunological, neurodegenerative and other age-related diseases, (iv) the impact of nutrition and lifestyle on buccal MN cytome assay biomarkers; (v) its potential for application to studies of DNA damage in children and obesity, and (vi) the growing prospects of enhancing the clinical utility by automated scoring of the buccal MN cytome assay biomarkers by image recognition software developed using artificial intelligence. The most important knowledge gap is the need of prospective studies to test whether the buccal MN cytome assay biomarkers predict health and disease. •Buccal micronucleus (MN) cytome assay in human exposure studies is reviewed.•Association of buccal MN cytome biomarkers with diseases is described.•Impact of nutrition and lifestyle on buccal MN cytome assay biomarkers is discussed.•Automated buccal MN cytome assay using artificial intelligence is proposed.•Prospective studies are needed to test if buccal MN frequency predicts disease risk.
ISSN:1383-5718
1879-3592
DOI:10.1016/j.mrgentox.2023.503724