Blue-light irradiation induced partial nitrification

• Published in: Water research (Oxford) 2024-05, Vol.254, p.121381-121381, Article 121381
• Main Authors: Zheng, Ru, Feng, Yiming, Kong, Lingrui, Wu, Xiaogang, Zhou, Jianhang, Zhang, Liguo, Liu, Sitong
• Format: Article
• Language: English
• Subjects: Blue light; Differentially expressed genes (DEGs); Nitrifiers; Partial nitrification; Photoreceptor protein
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2024.121381

•Partial nitrification was achieved under blue-light irradiation mainly through inspiring AOA.•Blue light enhanced TCA cycle, antioxidant defense and DNA repair capacity in AOA.•NOB was hindered under blue-light irradiation in via of BLUF photoreceptor protein.•AOB had stronger DNA repair capacity than NOB under blue-light irradiation. The role of ray radiation from the sunlight acting on organisms has long-term been investigated. However, how the light with different wavelengths affects nitrification and the involved nitrifiers are still elusive. Here, we found more than 60 % of differentially expressed genes (DEGs) in nitrifiers were observed under irradiation of blue light with wavelengths of 440–480 nm, which were 13.4 % and 20.3 % under red light and white light irradiation respectively. Blue light was more helpful to achieve partial nitrification rather than white light or red light, where ammonium oxidization by ammonia-oxidizing archaea (AOA) with the increased relative abundance from 8.6 % to 14.2 % played a vital role. This was further evidenced by the enhanced TCA cycle, reactive oxygen species (ROS) scavenge and DNA repair capacity in AOA under blue-light irradiation. In contrast, nitrite-oxidizing bacteria (NOB) was inhibited severely to achieve partial nitrification, and the newly discovered encoded blue light photoreceptor proteins made them more sensitive to blue light and hindered cell activity. Ammonia-oxidizing bacteria (AOB) expressed genes for DNA repair capacity under blue-light irradiation, which ensured their tiny impact by light irradiation. This study provided valuable insights into the photosensitivity mechanism of nitrifiers and shed light on the diverse regulatory by light with different radiation wavelengths in artificial systems, broadening our comprehension of the nitrogen cycle on earth. [Display omitted]