Photoinactivation by UVA radiation and visible light of Candida auris compared to other fungi

In addition to the rising number of patients affected by viruses and bacteria, the number of fungal infections has also been rising over the years. Due to the increase in resistance to various antimycotics, investigations into further disinfection options are important. In this study, two yeasts ( C...

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Bibliographic Details
Published in:Photochemical & photobiological sciences 2024-04, Vol.23 (4), p.681-692
Main Authors: Gierke, Anna-Maria, Hessling, Martin
Format: Article
Language:English
Subjects:
Antifungal Agents
Biochemistry
Biomaterials
Candida auris
Chemistry
Chemistry and Materials Science
Humans
Light
Microbial Sensitivity Tests
Original Papers
Photosensitizing Agents - pharmacology
Physical Chemistry
Plant Sciences
Saccharomyces cerevisiae
Ultraviolet Rays
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Summary:In addition to the rising number of patients affected by viruses and bacteria, the number of fungal infections has also been rising over the years. Due to the increase in resistance to various antimycotics, investigations into further disinfection options are important. In this study, two yeasts ( Candida auris and Saccharomyces cerevisiae ) and a mold ( Cladosporium cladosporioides ) were irradiated at 365, 400, and 450 nm individually. The resulting log 1 reduction doses were determined and compared with other studies. Furthermore, fluorescence measurements of C. auris were performed to detect possible involved photosensitizers. A roughly exponential photoinactivation was observed for all three fungi and all irradiation wavelengths with higher D90 doses for longer wavelengths. The determined log 1 reduction doses of C. auris and S. cerevisiae converged with increasing wavelength. However, S. cerevisiae was more photosensitive than C. auris for all irradiation wavelengths and is therefore not a suitable C. auris surrogate for photoinactivation experiments. For the mold C. cladosporioides , much higher D90 doses were determined than for both yeasts. Concerning potential photosensitizers, flavins and various porphyrins were detected by fluorescence measurements. By excitation at 365 nm, another, so far unreported fluorophore and potential photosensitizer was also observed. Based on its fluorescence spectrum, we assume it to be thiamine. Graphic abstract
ISSN:1474-905X
1474-9092
DOI:10.1007/s43630-024-00543-4