Transcription factor CsMYB36 regulates fruit neck length via mediating cell expansion in cucumber

• Published in: Plant physiology (Bethesda) 2024-05, Vol.195 (2), p.958-969
• Main Authors: Wang, Chunhua, Yao, Hongxin, Wang, Chen, Gao, Luyao, Chai, Xingwen, Fang, Kai, Du, Yalin, Hao, Ning, Cao, Jiajian, Wu, Tao
• Format: Article
• Language: English
• Subjects: Cucumis sativus - genetics; Cucumis sativus - growth & development; Fruit - genetics; Fruit - growth & development; Gene Expression Regulation, Plant; Plant Proteins - genetics; Plant Proteins - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1093/plphys/kiae140

The fruit neck is an important agronomic trait of cucumber (Cucumis sativus). However, the underlying genes and regulatory mechanisms involved in fruit neck development are poorly understood. We previously identified a cucumber yellow-green peel (ygp) mutant, whose causal gene is MYB DOMAIN PROTEIN 36 (CsMYB36). This study showed that the ygp mutant exhibited a shortened fruit neck and repressed cell expansion in the fruit neck. Further functional analysis showed that CsMYB36 was also a target gene, and its expression was enriched in the fruit neck. Overexpression of CsMYB36 in the ygp mutant rescued shortened fruit necks. Furthermore, transcriptome analysis and reverse transcription quantitative PCR (RT-qPCR) assays revealed that CsMYB36 positively regulates the expression of an expansin-like A3 (CsEXLA3) in the fruit neck, which is essential for cell expansion. Yeast 1-hybrid and dual-luciferase assays revealed that CsMYB36 regulates fruit neck elongation by directly binding to the promoter of CsEXLA3. Collectively, these findings demonstrate that CsMYB36 is an important gene in the regulation of fruit neck length in cucumber plants.