Behavior of humic acid as a micellar phase in micellar electrokinetic chromatography (MEKC)

The possibility of humic acids acting as micellar phase in micellar electrokinetic chromatography was evaluated. We investigated the separation of naphthalene in capillary electrophoresis using various samples of humic acids as micellar phase under different pH conditions, concentrations of humic ac...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2005-09, Vol.151 (1-2), p.115-122
Main Authors: DE MORAES, Solange Leite, REZENDE, Maria Olimpia
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:The possibility of humic acids acting as micellar phase in micellar electrokinetic chromatography was evaluated. We investigated the separation of naphthalene in capillary electrophoresis using various samples of humic acids as micellar phase under different pH conditions, concentrations of humic acid, and temperature. The humic acid samples studied were from different origins including peat, vermicompost and a commercial sample acquired from Aldrich. Methanol was used as a marker for the electroosmotic flow. The results indicate that the formation of micelle depends on the number and nature of the hydrophobic association sites in an aqueous humic acid solution and on the origin and concentration of the humic acid at a defined pH. At lower pH values, the possibility of the humic acid molecule forming pseudomicelles increases due to a combination of neutralized and dissociated charged sites.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-005-0383-y