Spatial and temporal resolution in cryo-electron microscopy—A scope for nano-chemistry

Cryo-electron microscopy has evolved in an established approach to study the structure of bio-colloids. Recent developments in instrumentation and automation, often demanded by life sciences, made cryo-EM a general tool in colloid chemistry. Recently improved instrumentation for vitrification has re...

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Bibliographic Details
Published in:Current opinion in colloid & interface science 2005-12, Vol.10 (5), p.245-249
Main Authors: Frederik, P.M., Sommerdijk, N.
Format: Article
Language:English
Subjects:
Chemistry
Colloidal state and disperse state
Cryo-EM
Exact sciences and technology
General and physical chemistry
Langmuir films
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Time resolution
Tomography
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Summary:Cryo-electron microscopy has evolved in an established approach to study the structure of bio-colloids. Recent developments in instrumentation and automation, often demanded by life sciences, made cryo-EM a general tool in colloid chemistry. Recently improved instrumentation for vitrification has resulted in reliable and reproducible preparation of specimen in water and other solvents. A dynamic approach, following processes in time is gaining importance and a time resolution up to 1 ms is expected to become general available. With improved instrumentation and automation 3D reconstructions by cryo-EM tomography are becoming routinely accessible. Tomography as such or in conjunction with time resolved microscopy is expected to give new insights in (macro)molecular assembly/disassembly mechanisms and thus become an essential tool in nano-chemistry.
ISSN:1359-0294
1879-0399
DOI:10.1016/j.cocis.2005.09.018