Computer-assisted modeling, prediction, and multifactor optimization of hydrophobic compounds in liquid chromatography

The traditional overlapping resolution mapping (ORM) scheme was modified for application in HPLC with the flow rate of the mobile phase as a new factor. The mobile phase composition and a flow rate were taken as parameters to influence effective resolution and elution time. Chromatograms were obtain...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2005-11, Vol.152 (1-2), p.113-121
Main Authors: YU PING ZHANG, WEN JUN GONG, KWANG PILL LEE, SEONG HO CHOI, GOPALAN, Anantha Tyengar, ZHUO BIN YUAN
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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Summary:The traditional overlapping resolution mapping (ORM) scheme was modified for application in HPLC with the flow rate of the mobile phase as a new factor. The mobile phase composition and a flow rate were taken as parameters to influence effective resolution and elution time. Chromatograms were obtained for the separation of the 16 compounds for ten trial conditions. Computer-assisted mathematical simulation was used to obtain optimum conditions for resolution and separation time of the compounds. The validity of the model developed was confirmed by experiments.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-005-0419-3