Detection of d-serine in neural samples by saccharide enhanced chiral capillary electrophoresis

Highly fluorescent enantiomeric derivatives of d/ l-serine from naphthalene-2,3-dicarboxaldehyde were resolved by hydroxypropyl-γ-cyclodextrin modified capillary electrophoresis using a saccharide as an enhancing chiral selector. Four saccharides, i.e. d-(+)-glucose, d-(−)-fructose, β-lactose, and s...

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Bibliographic Details
Published in:Analytica chimica acta 2005-01, Vol.528 (1), p.101-106
Main Authors: Quan, Zhe, Song, Yaru, Feng, Yangzheng, LeBlanc, Michael H., Liu, Yi-Ming
Format: Article
Language:English
Subjects:
Analytical chemistry
Aplysia
Aplysia ganglia
Capillary electrophoresis
Chemistry
Chromatographic methods and physical methods associated with chromatography
d-Serine
Enantiomeric separation
Exact sciences and technology
Microdialysates
Other chromatographic methods
Rat brain tissue
Spectrometric and optical methods
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Summary:Highly fluorescent enantiomeric derivatives of d/ l-serine from naphthalene-2,3-dicarboxaldehyde were resolved by hydroxypropyl-γ-cyclodextrin modified capillary electrophoresis using a saccharide as an enhancing chiral selector. Four saccharides, i.e. d-(+)-glucose, d-(−)-fructose, β-lactose, and sucrose were tested. Similar enhancing effects were observed. Coupled with laser induced fluorescence detection, this separation was applied to the determination of d-serine in neural samples including rat brain tissues, Aplysia ganglia, microdialysates from rat brain, and Aplysia individual neurons. High levels of d-serine were found in certain rat brain sections including hippocampus and striatum. d-Serine was also found to occur in Aplysia ganglia, but interestingly, it was not detected in single neurons isolated from Aplysia ganglia.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2004.10.020