Immunosensors for pollutants working in organic media. Study of performances of different tracers with luminescent detection

A comparative study of enzymatic and non-enzymatic labels combined with luminescence detection, developed for immunosensing of pesticide residues (carbaryl, 1-naphthol, irgarol 1051) in organic media, is presented. Peroxidase and alkaline phosphatase enzymes with fluorogenic (3-p-hydroxyphenylpropan...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2006-04, Vol.384 (7-8), p.1540-1547
Main Authors: González-Martínez, M A, Penalva, J, Rodríguez-Urbis, J C, Brunet, E, Maquieira, A, Puchades, R
Format: Article
Language:English
Subjects:
Adamantane - analogs & derivatives
Adamantane - chemistry
Caffeic Acids - chemistry
Carbaryl - chemistry
Chemistry, Organic - methods
Hazardous Substances - analysis
Immunochemistry - methods
Indicators and Reagents
Luminescence
Models, Chemical
Naphthols - pharmacology
Organic Chemicals
Peroxidases - chemistry
Spectrometry, Fluorescence - methods
Terbium - pharmacology
Triazines - pharmacology
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Summary:A comparative study of enzymatic and non-enzymatic labels combined with luminescence detection, developed for immunosensing of pesticide residues (carbaryl, 1-naphthol, irgarol 1051) in organic media, is presented. Peroxidase and alkaline phosphatase enzymes with fluorogenic (3-p-hydroxyphenylpropanoic acid) and luminogenic (AMPPD derivative) substrates, respectively, were assessed as enzymatic markers. As an alternative, terbium(III) chelate, with time-resolved fluorescence detection, was evaluated as a non-enzymatic label. The best sensitivity was achieved by use of alkaline phosphatase in an immunocomplex capture assay format (I (50) values 0.06, 0.27, and 7.45 microg L(-1) in buffer, 1:1 methanol-buffer, and methanol, respectively). Results were also good (I (50) 1.00 and 6.30 microg L(-1) for water and aqueous-organic mixture, respectively) for Tb(III) chelate in an immobilized conjugate assay format. Use of alkaline phosphatase label to measure carbaryl (100 ng L(-1)) in different spiked river water samples, after solid-phase extraction and analyte elution with an ethyl acetate-methanol mixture, resulted in recoveries ranging from 81 to 98%, with acceptable precision (CV 4-14%, n=4).
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-0306-8