Functionalization and magnetonavigation of T-lymphocytes functionalized via nanocomposite capsules targeting with electromagnetic tweezers

• Published in: Nanomedicine 2024-04, Vol.57, p.102742-102742, Article 102742
• Main Authors: Abalymov, Anatolii, Kurochkin, Maxim A., German, Sergei, Komlev, Aleksei, Vavaev, Evgeny S., Lyubin, Evgeny V., Fedyanin, Andrey A., Gorin, Dmitry, Novoselova, Marina
• Format: Article
• Language: English
• Subjects: Cell delivery; Drug delivery systems; Freezing-induced loading; Layer-by-layer; T-lymphocytes
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2024.102742

Modification of T-lymphocytes, which are capable of paracellular transmigration is a promising trend in modern personalized medicine. However, the delivery of required concentrations of functionalized T-cells to the target tissues remains a problem. We describe a novel method to functionalize T-cells with magnetic nanocapsules and target them with electromagnetic tweezers. T-cells were modified with the following magnetic capsules: Parg/DEX (150 nm), BSA/TA (300 nm), and BSA/TA (500 nm). T-cells were magnetonavigated in a phantom blood vessel capillary in cultural medium and in whole blood. The permeability of tumor tissues to captured T-cells was analyzed by magnetic delivery of modified T-cells to spheroids formed from 4T1 breast cancer cells. The dynamics of T-cell motion under a magnetic field gradient in model environments were analyzed by particle image velocimetry. The magnetic properties of the nanocomposite capsules and magnetic T-cells were measured. The obtained results are promising for biomedical applications in cancer immunotherapy. We present a method for modifying T-lymphocytes using magnetic nanocomposite capsules and targeting them with electromagnetic tweezers. By integrating magnetic capsules (Parg/DEX, BSA/TA) into Jurkat cells, we achieved successful magnetic navigation in simulated blood vessels. The captured T-cells were magnetically delivered to tumor spheroids, allowing assessment of tumor tissue permeability to captured T-cells. Our results show promising applications in cancer immunotherapy, highlighting the potential of this approach for personalized medicine. [Display omitted]