l-carnitine enhances the kinematics and protects the sperm membranes of chilled and frozen-thawed Peruvian Paso horse spermatozoa

l-carnitine (LC) transports fatty acids to the mitochondria for energy production, reducing lipid availability for peroxidation through β-oxidation. This research examines the effect of LC supplementation to two skimmed milk-based extenders on the cryosurvival of chilled (5°C) and frozen-thawed Peru...

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Bibliographic Details
Published in:Cryobiology 2024-06, Vol.115, p.104884-104884, Article 104884
Main Authors: Palacios, Paula, Peláez, Gabriela, Soria, Manuel, Méndez, Silvana, Galarza-Álvarez, Luis, Dorado, Jesús, Santiago-Moreno, Julián, Galarza, Diego A.
Format: Article
Language:English
Subjects:
Cryopreservation
l-carnitine
Peruvian Paso horse
Spermatozoa
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Summary:l-carnitine (LC) transports fatty acids to the mitochondria for energy production, reducing lipid availability for peroxidation through β-oxidation. This research examines the effect of LC supplementation to two skimmed milk-based extenders on the cryosurvival of chilled (5°C) and frozen-thawed Peruvian Paso horse spermatozoa .An initial experiment determined the optimal LC concentration (0, 1, 5, 10, 25, and 50 mM) when added to INRA-96® and UHT (skimmed milk + 6% egg yolk) extenders, using nine ejaculates from three stallions chilled for up to 96 h. Subsequently, the effect of 25 mM LC supplementation (the optimal concentration) on chilling (INRA-96) and freezing (INRA-Freeze®) extenders was evaluated using eight pooled samples from sixteen ejaculates (2 ejaculates/pool) from four stallions. Results indicated that all LC concentrations produced significantly higher values (P
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2024.104884