Combined transcriptomics and metabolomics to analyse the response of Cuminum cyminum L. under Pb stress

• Published in: The Science of the total environment 2024-05, Vol.923, p.171497-171497, Article 171497
• Main Authors: Yang, Xinlong, Chen, Yinguang, Liu, Weiguo, Huang, Tingwen, Yang, Yang, Mao, Yuqing, Meng, Yao
• Format: Article
• Language: English
• Subjects: Cumin; Lead stress; Metabolome; Transcriptome
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.171497

Lead (Pb) can disrupt plant gene expression, modify metabolite contents, and influence the growth of plants. Cuminum cyminum L. is highly adaptable to adversity, but molecular mechanism by which it responds to Pb stress is unknown. For this study, transcriptomic and metabolomic sequencing was performed on root tissues of C. cyminum under Pb stress. Our results showed that high Pb stress increased the activity of peroxidase (POD), the contents of malondialdehyde (MDA) and proline by 80.03 %, 174.46 % and 71.24 %, respectively. Meanwhile, Pb stress decreased the activities of superoxide dismutase (SOD) and catalase (CAT) as well as contents of soluble sugars and GSH, which thus affected the growth of C. cyminum. In addition, Pb stress influenced the accumulation and transport of Pb in C. cyminum. Metabolomic results showed that Pb stress affected eight metabolic pathways involving 108 differentially expressed metabolites, primarily amino acids, organic acids, and carbohydrates. The differentially expressed genes identified through transcriptome analysis were mainly involved the oxidation reductase activity, transmembrane transport, phytohormone signaling, and MAPK signaling pathway. The results of this study will help to understand the molecular mechanisms of C. cyminum response to Pb stress, and provide a basis for screening seeds with strong resistance to heavy metals. [Display omitted] •Pb stress shaped low translocation of Pb from roots to seeds.•Pb stress decreased soluble sugar content as well as SOD and CAT activities.•Amino acids, organic acids, and carbohydrates are essential in response to Pb stress.•Pb disrupted plant hormone signal transduction and glutathione metabolic pathways.