Kinesin-7 CENP-E mediates chromosome alignment and spindle assembly checkpoint in meiosis I

In eukaryotes, meiosis is the genetic basis for sexual reproduction, which is important for chromosome stability and species evolution. The defects in meiosis usually lead to chromosome aneuploidy, reduced gamete number, and genetic diseases, but the pathogenic mechanisms are not well clarified. Kin...

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Bibliographic Details
Published in:Chromosoma 2024-04, Vol.133 (2), p.149-168
Main Authors: Zhang, Jing-Lian, Xu, Meng-Fei, Chen, Jie, Wei, Ya-Lan, She, Zhen-Yu
Format: Article
Language:English
Subjects:
Anaphase
Aneuploidy
Animal Genetics and Genomics
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cell division
Chromosomes
Developmental Biology
E protein
Eukaryotic Microbiology
Genetic disorders
Human Genetics
Kinesin
Life Sciences
Meiosis
Metaphase
Sexual reproduction
Sperm
Spermatocytes
Spermatogenesis
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Summary:In eukaryotes, meiosis is the genetic basis for sexual reproduction, which is important for chromosome stability and species evolution. The defects in meiosis usually lead to chromosome aneuploidy, reduced gamete number, and genetic diseases, but the pathogenic mechanisms are not well clarified. Kinesin-7 CENP-E is a key regulator in chromosome alignment and spindle assembly checkpoint in cell division. However, the functions and mechanisms of CENP-E in male meiosis remain largely unknown. In this study, we have revealed that the CENP-E gene was highly expressed in the rat testis. CENP-E inhibition influences chromosome alignment and spindle organization in metaphase I spermatocytes. We have found that a portion of misaligned homologous chromosomes is located at the spindle poles after CENP-E inhibition, which further activates the spindle assembly checkpoint during the metaphase-to-anaphase transition in rat spermatocytes. Furthermore, CENP-E depletion leads to abnormal spermatogenesis, reduced sperm count, and abnormal sperm head structure. Our findings have elucidated that CENP-E is essential for homologous chromosome alignment and spindle assembly checkpoint in spermatocytes, which further contribute to chromosome stability and sperm cell quality during spermatogenesis.
ISSN:0009-5915
1432-0886
1432-0886
DOI:10.1007/s00412-024-00818-w