Immunophenotypic characterization of leukemic stem cells in acute myeloid leukemia using single tube 10‐colour panel by multiparametric flow cytometry: Deciphering the spectrum, complexity and immunophenotypic heterogeneity

Introduction Despite extensive research, comprehensive characterization of leukaemic stem cells (LSC) and information on their immunophenotypic differences from normal haematopoietic stem cells (HSC) is lacking. Herein, we attempted to unravel the immunophenotypic (IPT) characteristics and heterogen...

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Bibliographic Details
Published in:International journal of laboratory hematology 2024-08, Vol.46 (4), p.646-656
Main Authors: Das, Nupur, Panda, Devasis, Gajendra, Smeeta, Gupta, Ritu, Thakral, Deepshi, Kaur, Gurvinder, Khan, Aafreen, Singh, Vivek Kumar, Vemprala, Arushi, Bakhshi, Sameer, Seth, Rachna, Sahoo, Ranjit Kumar, Sharma, Atul, Rai, Sandeep, Prajapati, Vijay K., Singh, Saroj
Format: Article
Language:English
Subjects:
acute myeloid leukaemia
Acute myeloid leukemia
Antigens
Bone marrow
CD11b antigen
CD123 antigen
CD22 antigen
CD25 antigen
CD34 antigen
CD38 antigen
CD44 antigen
CD45 antigen
CD45RA antigen
CD56 antigen
CD7 antigen
Chronic lymphocytic leukemia
Flow cytometry
Hematopoietic stem cells
leukaemic stem cells
single cell sequencing
Stem cells
Transcriptomes
Transcriptomics
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Summary:Introduction Despite extensive research, comprehensive characterization of leukaemic stem cells (LSC) and information on their immunophenotypic differences from normal haematopoietic stem cells (HSC) is lacking. Herein, we attempted to unravel the immunophenotypic (IPT) characteristics and heterogeneity of LSC using multiparametric flow cytometry (MFC) and single‐cell sequencing. Materials and Methods Bone marrow aspirate samples from patients with acute myeloid leukaemia (AML) were evaluated using MFC at diagnostic and post induction time points using a single tube‐10‐colour‐panel containing LSC‐associated antibodies CD123, CD45RA, CD44, CD33 and COMPOSITE (CLL‐1, TIM‐3, CD25, CD11b, CD22, CD7, CD56) with backbone markers that is, CD45, CD34, CD38, CD117, sCD3. Single‐cell sequencing of the whole transcriptome was also done in a bone marrow sample. Results LSCs and HSCs were identified in 225/255 (88.2%) and 183/255 (71.6%) samples, respectively. Significantly higher expression was noted for COMPOSITE, CD45RA, CD123, CD33, and CD44 in LSCs than HSCs (p 
ISSN:1751-5521
1751-553X
1751-553X
DOI:10.1111/ijlh.14250