Toward the boosted loading of cisplatin drug into liposome nanocarriers

• Published in: European journal of pharmaceutics and biopharmaceutics 2024-05, Vol.198, p.114245-114245, Article 114245
• Main Authors: Wróblewska, Anna M., Łukawska, Ewelina, Wakuła, Zuzanna, Zajda, Joanna, Keppler, Bernhard K., Timerbaev, Andrei R., Matczuk, Magdalena
• Format: Article
• Language: English
• Subjects: Cisplatin; Delivery system; Drug loading efficiency; Hyphenated techniques; Liposome
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2024.114245

[Display omitted] Current challenges in oncology are largely associated with the need to improve the effectiveness of cancer treatment and to reduce drug’s side effects. An effective strategy to cope with these challenges is behind designing and developing drug delivery systems based on smart nanomaterials and approved anticancer drugs. The present study offers a novel and straightforward approach to efficiently load the cisplatin drug into the newly constructed liposome-based nanosystems as well a reliable technique for monitoring this process based on capillary electrophoresis hyphenated with inductively coupled plasma tandem mass spectrometry. The proposed drug-loading methodology comprises liposome formation via a simple ethanol-injection method and propels increased drug encapsulation using tailor-made freeze-thawing or lyophilization-hydration procedures. To optimize liposome generation and drug encapsulation, the effects of dilution medium and liposome composition (types of phospholipids and their percentage ratio) have been investigated in detail. It was shown that modest alterations of the composition of three-component phospholipid liposomes and parameters of the freeze-thawing procedure have a strong impact on the formation of cisplatin–liposome systems. The obtained cisplatin–liposome formulation features a remarkable degree of drug encapsulation, over 100 mg L–1, and holds promise for further preclinical development as a potent drug-delivery platform.