Determination of fluoroacetic acid in water and biological samples by GC-FID and GC-MS in combination with solid-phase microextraction

A novel procedure has been developed for determination of fluoroacetic acid (FAA) in water and biological samples. It involves ethylation of FAA with ethanol in the presence of sulfuric acid, solid-phase microextraction of the ethyl fluoroacetate formed, and subsequent analysis by GC-FID or by GC-MS...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2006-11, Vol.386 (5), p.1395-1400
Main Authors: Koryagina, Nadezhda L, Savelieva, Elena I, Khlebnikova, Natalia S, Goncharov, Nikolay V, Jenkins, Richard O, Radilov, Andrey S
Format: Article
Language:English
Subjects:
Animals
Biological analysis
Biological properties
Biological samples
Blood plasma
Brain - metabolism
Chromatography, Gas - methods
Contamination
Detection limits
Ethanol
Flame Ionization - methods
Fluoroacetates - administration & dosage
Fluoroacetates - analysis
Fluoroacetic acid
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
Heart
Kidney - chemistry
Kidney - metabolism
Liver - chemistry
Liver - metabolism
Plasma
Plasma - chemistry
Rabbits
Rats
Rats, Wistar
Reproducibility of Results
Sensitivity and Specificity
Solid phase methods
Solid Phase Microextraction - instrumentation
Solid Phase Microextraction - methods
Solid phases
Sulfuric acid
Tissue Distribution
Water - chemistry
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Summary:A novel procedure has been developed for determination of fluoroacetic acid (FAA) in water and biological samples. It involves ethylation of FAA with ethanol in the presence of sulfuric acid, solid-phase microextraction of the ethyl fluoroacetate formed, and subsequent analysis by GC-FID or by GC-MS in selected-ion-monitoring mode. The detection limits for FAA in water, blood plasma, and organ homogenates are 0.001 microg mL(-1), 0.01 microg mL(-1), and 0.01 microg g(-1), respectively. The determination error at concentrations close to the detection limit was less than 50%. For analysis of biological samples, the approach has the advantages of overcoming the matrix effect and protecting the GC and GC-MS systems from contamination. Application of the approach to determination of FAA in blood plasma and organ tissues of animals poisoned with sodium fluoroacetate reveals substantial differences between the dynamics of FAA accumulation and clearance in rabbits and rats.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-0713-x